uPA Receptor
HUABIO
catalog: HA601039
mouse monoclonal (A6E8)
reactivity: human
application: western blot

Western blot analysis of uPA Receptor on different lysates with Mouse anti-uPA Receptor antibody (HA601039) at 1/1,000 dilution. Lane 1: U937 cell lysate Lane 2: U937 treated with 200 nM PMA for 72 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 37-60 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601039) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
uPA Receptor
HUABIO
catalog: HA601040
mouse monoclonal (A6E7)
reactivity: human
application: western blot

Western blot analysis of uPA Receptor on different lysates with Mouse anti-uPA Receptor antibody (HA601040) at 1/500 dilution. Lane 1: U937 cell lysate Lane 2: U937 cell lysate treated with 200 nM PMA for 72 hours Lysates/proteins at 10 µg/Lane. Predicted band size: 36 kDa Observed band size: 36~60 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601040) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
uPA Receptor
HUABIO
catalog: HA601296
mouse monoclonal (A6E8-R)
reactivity: human
application: western blot

Western blot analysis of uPA Receptor on different lysates with Mouse anti-uPA Receptor antibody (HA601296) at 1/1,000 dilution. Lane 1: U937 cell lysate Lane 2: U937 treated with 200 nM PMA for 72 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 37-60 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601296) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
Human uPAR
HUABIO
catalog: HA601437
mouse monoclonal (PSH13-76)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human uPAR matched pair antibodies Capture: HA601437, Human uPAR Rabbit mAb [PSH13-76] Detector: HA601438, Human uPAR Rabbit mAb [PSH13-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601437) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human uPAR protein (HA211102) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA601438, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μg
price: 649.00 USD
to the supplier
Human uPAR
HUABIO
catalog: HA601438
mouse monoclonal (PSH13-77)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human uPAR matched pair antibodies Capture: HA601437, Human uPAR Rabbit mAb [PSH13-76] Detector: HA601438, Human uPAR Rabbit mAb [PSH13-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601437) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human uPAR protein (HA211102) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA601438, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μg
price: 649.00 USD
to the supplier
Human uPAR
HUABIO
catalog: HA601439B
mouse monoclonal (PSH13-77)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human uPAR matched pair antibodies Capture: HA601437, Human uPAR Rabbit mAb [PSH13-76] Detector: HA601438, Human uPAR Rabbit mAb [PSH13-77] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601437) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human uPAR protein (HA211102) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA601438, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
uPA Receptor
HUABIO
catalog: HA610169
mouse monoclonal (A6E8-R)
reactivity: human
application: western blot

Western blot analysis of uPA Receptor on different lysates with Mouse anti-uPA Receptor antibody (HA610169) at 1/1,000 dilution. Lane 1: U937 cell lysate Lane 2: U937 treated with 200 nM PMA for 72 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 37-60 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610169) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μg
price: 649.00 USD
to the supplier