Tissue type plasminogen activator
HUABIO
catalog: ET1702-19
domestic rabbit monoclonal (JF0958)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tissue type plasminogen activator antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tissue type plasminogen activator antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-19, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Human tPA
HUABIO
catalog: HA725115
domestic rabbit monoclonal (PSH13-10)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human tPA matched pair antibodies Capture: HA725115, Human tPA Rabbit mAb [PSH13-10] Detector: HA725116, Human tPA Rabbit mAb [PSH13-11] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725115) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human tPA protein (HA211026) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA725116, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native tPA in HUVEC cell supernatant Capture: HA725115, Human tPA Rabbit mAb [PSH13-10] Detector: HA725116, Human tPA Rabbit mAb [PSH13-11] Interpolated concentration of native tPA was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean tPA concentration was determined to be 17124 pg/mL in HUVEC cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human tPA
HUABIO
catalog: HA725116
domestic rabbit monoclonal (PSH13-11)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human tPA matched pair antibodies Capture: HA725115, Human tPA Rabbit mAb [PSH13-10] Detector: HA725116, Human tPA Rabbit mAb [PSH13-11] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725115) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human tPA protein (HA211026) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA725116, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native tPA in HUVEC cell supernatant Capture: HA725115, Human tPA Rabbit mAb [PSH13-10] Detector: HA725116, Human tPA Rabbit mAb [PSH13-11] Interpolated concentration of native tPA was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean tPA concentration was determined to be 17124 pg/mL in HUVEC cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human tPA
HUABIO
catalog: HA725117B
domestic rabbit monoclonal (PSH13-11)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human tPA matched pair antibodies Capture: HA725115, Human tPA Rabbit mAb [PSH13-10] Detector: HA725116, Human tPA Rabbit mAb [PSH13-11] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725115) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human tPA protein (HA211026) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA725116, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native tPA in HUVEC cell supernatant Capture: HA725115, Human tPA Rabbit mAb [PSH13-10] Detector: HA725116, Human tPA Rabbit mAb [PSH13-11] Interpolated concentration of native tPA was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean tPA concentration was determined to be 17124 pg/mL in HUVEC cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier
Human tPA
HUABIO
catalog: HA725117H
domestic rabbit monoclonal (PSH13-11)
reactivity: human
conjugate: HRP
application: ELISA

Sandwich ELISA analysis of Human tPA matched pair antibodies Capture: HA725115, Human tPA Rabbit mAb [PSH13-10] Detector: HA725116, Human tPA Rabbit mAb [PSH13-11] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725115) diluted in carbonate/bicarbonate buffer, at a concentration of 0.5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human tPA protein (HA211026) starting from 100,000 pg/ml to 0 pg/ml and detect antibody (HA725116, HRP, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier
Tissue type plasminogen activator
HUABIO
catalog: HA750338
domestic rabbit monoclonal (JF0958)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tissue type plasminogen activator antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750338, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tissue type plasminogen activator antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750338, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier