domestic rabbit monoclonal (PSH07-16)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722804) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722804) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722804) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722804) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-56)
reactivity: human, mouse
application: western blot, dot blot
reactivity: human, mouse
application: western blot, dot blot
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722849) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722849) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722849) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722849) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Dot blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different proteins with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA722849) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Cytosolic Phospholipase A2 (negative) Lane 2: Phospho-Cytosolic Phospholipase A2 (S505) (positive) Proteins loading: 50ng, 25ng, 5ng, 1ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 6 seconds; ECL: K1801.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-16)
reactivity: human, mouse
application: western blot
reactivity: human, mouse
application: western blot
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751137) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751137) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751137) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751137) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-56)
reactivity: human, mouse
application: western blot, dot blot
reactivity: human, mouse
application: western blot, dot blot
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751176) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751176) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on RAW264.7 cell lysates with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751176) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751176) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Dot blot analysis of Phospho-Cytosolic Phospholipase A2 (S505) on different proteins with Rabbit anti-Phospho-Cytosolic Phospholipase A2 (S505) antibody (HA751176) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified Cytosolic Phospholipase A2 (negative) Lane 2: Phospho-Cytosolic Phospholipase A2 (S505) (positive) Proteins loading: 50ng, 25ng, 5ng, 1ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 6 seconds; ECL: K1801.
quantity: 100μl
price: 649.00 USD
to the supplier