HUABIO PECAM 1 antibody

CD31
HUABIO
catalog: ER31219

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD31 on different lysates with Rabbit anti-CD31 antibody (ER31219) at 1/1,000 dilution. Lane 1: THP-1 cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (negative) (10 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 83 kDa Observed band size: 120-130 kDa Exposure time: Lane 1: 16 seconds; Lane 2-4: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31219) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-CD31 antibody (ER31219) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31219) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD31 antibody (ER31219) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER31219) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier

CD31
HUABIO
catalog: ET1608-48-50UL

domestic rabbit monoclonal (SU03-59)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD31 on THP-1 cell lysates with Rabbit anti-CD31 antibody (ET1608-48) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-48) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

CD31 was immunoprecipitated in 0.2mg THP-1 cell lysate with ET1608-48 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1608-48 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input) Lane 2: Rabbit IgG instead of ET1608-48 in THP-1 cell lysate Lane 3: ET1608-48 IP in THP-1 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD31 antibody (ET1608-48) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-48) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier

CD31
HUABIO
catalog: HA601240

mouse monoclonal (7-A1-R)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD31 on different lysates with Mouse anti-CD31 antibody (HA601240) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601240) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling CD31 with Mouse anti-CD31 antibody (HA601240) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD31 antibody (HA601240) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD31 antibody (HA601240) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601240) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

CD31
HUABIO
catalog: HA610115

mouse monoclonal (7-A1-R)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD31 on different lysates with Mouse anti-CD31 antibody (HA610115) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610115) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling CD31 with Mouse anti-CD31 antibody (HA610115) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD31 antibody (HA610115) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD31 antibody (HA610115) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610115) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier

CD31
HUABIO
catalog: HA720220F

domestic rabbit monoclonal (SU03-59)
reactivity: human

Immunofluorescence analysis of paraffin-embedded human tonsils tissue labeling CD31 with Rabbit anti-CD31 antibody (HA720220F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA720220F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 429.00 USD
to the supplier

Human CD31
HUABIO
catalog: HA722906

domestic rabbit monoclonal (PSH07-82)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CD31 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722906) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD31 protein (HA210891) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA722907, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD31in human serum samples. The concentrations of CD31 were interpolated from the CD31 standard curve and corrected for sample dilution. Undiluted samples are human serum 20%. The mean CD31 concentration was determined to be 39.7 ng/mL in human serum.

Interpolated concentrations of native CD31 in THP-1 and HeLa extract samples based on a 1,000 µg/ml extract load. The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curve and corrected for sample dilution. Undiluted samples are THP-1 extract 3% and HeLa extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 68.19 ng/ml in THP-1 extract and undetectable in HeLa extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Human CD31
HUABIO
catalog: HA722907

domestic rabbit monoclonal (PSH07-83)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human CD31 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722906) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD31 protein (HA210891) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA722907, Biotin, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD31in human serum samples. The concentrations of CD31 were interpolated from the CD31 standard curve and corrected for sample dilution. Undiluted samples are human serum 20%. The mean CD31 concentration was determined to be 39.7 ng/mL in human serum.

Interpolated concentrations of native CD31 in THP-1 and HeLa extract samples based on a 1,000 µg/ml extract load. The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curve and corrected for sample dilution. Undiluted samples are THP-1 extract 3% and HeLa extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 68.19 ng/ml in THP-1 extract and undetectable in HeLa extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Human CD31
HUABIO
catalog: HA722908B

domestic rabbit monoclonal (PSH07-83)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human CD31 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722906) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human CD31 protein (HA210891) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA722908B, 0.1 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD31in human serum samples. The concentrations of CD31 were interpolated from the CD31 standard curve and corrected for sample dilution. Undiluted samples are human serum 20%. The mean CD31 concentration was determined to be 39.7 ng/mL in human serum.

Interpolated concentrations of native CD31 in THP-1 and HeLa extract samples based on a 1,000 µg/ml extract load. The concentrations of CD31 were measured in duplicates, interpolated from the CD31 standard curve and corrected for sample dilution. Undiluted samples are THP-1 extract 3% and HeLa extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD31 concentration was determined to be 68.19 ng/ml in THP-1 extract and undetectable in HeLa extract.
quantity: 100μl
price: 409.00 USD
to the supplier

CD31
HUABIO
catalog: HA750151

domestic rabbit monoclonal (SU03-59)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section