domestic rabbit monoclonal (PSH09-53)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (HA723115) at 1/5,000 dilution. Lane 1: mouse eyeball tissue lysate (40 µg/Lane) Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane) Lane 3: rat eyeball tissue lysate (40 µg/Lane) Lane 4: rat liver tissue lysate (negative) (40 µg/Lane) Predicted band size: 28 kDa Observed band size: 33 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723115) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH09-54)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (HA723116) at 1/2,000 dilution. Lane 1: mouse eyeball tissue lysate (40 µg/Lane) Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane) Lane 3: rat eyeball tissue lysate (40 µg/Lane) Lane 4: rat liver tissue lysate (negative) (40 µg/Lane) Predicted band size: 28 kDa Observed band size: 33 kDa Exposure time: 3 minutes ; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723116) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA723116) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723116) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA723116) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723116) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH09-53)
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (HA751300) at 1/5,000 dilution. Lane 1: mouse eyeball tissue lysate (40 µg/Lane) Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane) Lane 3: rat eyeball tissue lysate (40 µg/Lane) Lane 4: rat liver tissue lysate (negative) (40 µg/Lane) Predicted band size: 28 kDa Observed band size: 33 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751300) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH09-54)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of PHOS / PDC on different lysates with Rabbit anti-PHOS / PDC antibody (HA751301) at 1/2,000 dilution. Lane 1: mouse eyeball tissue lysate (40 µg/Lane) Lane 2: mouse liver tissue lysate (negative) (40 µg/Lane) Lane 3: rat eyeball tissue lysate (40 µg/Lane) Lane 4: rat liver tissue lysate (negative) (40 µg/Lane) Predicted band size: 28 kDa Observed band size: 33 kDa Exposure time: 3 minutes ; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751301) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA751301) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751301) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat eyeball tissue with Rabbit anti-PHOS / PDC antibody (HA751301) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751301) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier