domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, ELISA, immunohistochemistry - paraffin section
Western blot analysis of IL-22 on IL22 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-85) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-22 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-85) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH03-11)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human IL-22 matched pair antibodies Capture: HA721939, Mouse CRP Rabbit mAb [PSH03-11] Detector: HA721940, Mouse CRP Rabbit mAb [PSH03-12] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721939) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-22 protein (HA210691) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721940, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Conditioned media was harvested after 48 hours. IL-22 was measured in 100% unstimulated and PHA stimulated PBMC supernatant.The concentrations of IL-22 were interpolated from the IL-22 standard curves and corrected for sample dilution.The mean IL-22 concentration was determined to be 79.7 pg/mL in PHA stimulated PBMC supernatant and there was no detectable signal in unstimulated supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-12)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human IL-22 matched pair antibodies Capture: HA721939, Mouse CRP Rabbit mAb [PSH03-11] Detector: HA721940, Mouse CRP Rabbit mAb [PSH03-12] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721939) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-22 protein (HA210691) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721940, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Conditioned media was harvested after 48 hours. IL-22 was measured in 100% unstimulated and PHA stimulated PBMC supernatant.The concentrations of IL-22 were interpolated from the IL-22 standard curves and corrected for sample dilution.The mean IL-22 concentration was determined to be 79.7 pg/mL in PHA stimulated PBMC supernatant and there was no detectable signal in unstimulated supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-12)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human IL-22 matched pair antibodies Capture: HA721939, Mouse CRP Rabbit mAb [PSH03-11] Detector: HA721940, Mouse CRP Rabbit mAb [PSH03-12] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721939) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-22 protein (HA210691) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA721940, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Conditioned media was harvested after 48 hours. IL-22 was measured in 100% unstimulated and PHA stimulated PBMC supernatant.The concentrations of IL-22 were interpolated from the IL-22 standard curves and corrected for sample dilution.The mean IL-22 concentration was determined to be 79.7 pg/mL in PHA stimulated PBMC supernatant and there was no detectable signal in unstimulated supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier