Human Oxytocin-neurophysin 1
HUABIO
catalog: HA723452
domestic rabbit monoclonal (PSH12-28)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Oxytocin-neurophysin 1 matched pair antibodies Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723452) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Oxytocin-neurophysin 1 protein (HA211134) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723453, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Oxytocin-neurophysin 1 in human serum samples. Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] Interpolated concentration of native Oxytocin-neurophysin 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Oxytocin-neurophysin 1 concentration was determined to be 1368 pg/mL in Human serum.

Interpolated concentrations of spiked Oxytocin-neurophysin 1 in cell culture media samples. Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] The concentrations of Oxytocin-neurophysin 1 were measured in duplicates, interpolated from the Oxytocin-neurophysin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human Oxytocin-neurophysin 1
HUABIO
catalog: HA723453
domestic rabbit monoclonal (PSH12-29)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Oxytocin-neurophysin 1 matched pair antibodies Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723452) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Oxytocin-neurophysin 1 protein (HA211134) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723453, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Oxytocin-neurophysin 1 in human serum samples. Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] Interpolated concentration of native Oxytocin-neurophysin 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Oxytocin-neurophysin 1 concentration was determined to be 1368 pg/mL in Human serum.

Interpolated concentrations of spiked Oxytocin-neurophysin 1 in cell culture media samples. Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] The concentrations of Oxytocin-neurophysin 1 were measured in duplicates, interpolated from the Oxytocin-neurophysin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human Oxytocin-neurophysin 1
HUABIO
catalog: HA723454B
domestic rabbit monoclonal (PSH12-29)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human Oxytocin-neurophysin 1 matched pair antibodies Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723452) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Oxytocin-neurophysin 1 protein (HA211134) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723453, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Oxytocin-neurophysin 1 in human serum samples. Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] Interpolated concentration of native Oxytocin-neurophysin 1 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Oxytocin-neurophysin 1 concentration was determined to be 1368 pg/mL in Human serum.

Interpolated concentrations of spiked Oxytocin-neurophysin 1 in cell culture media samples. Capture: HA723452, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-28] Detector: HA723453, Human Oxytocin-neurophysin 1 Rabbit mAb [PSH12-29] The concentrations of Oxytocin-neurophysin 1 were measured in duplicates, interpolated from the Oxytocin-neurophysin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier