Osteoprotegerin
HUABIO
catalog: EM1701-98

mouse monoclonal (9C1)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Osteoprotegerin on different lysates with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/2,000 dilution. Lane 1: MG-63 cell lysate Lane 2: Human liver tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat heart tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-98) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-98) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry analysis of 293 cells labeling Osteoprotegerin with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Osteoprotegerin antibody (EM1701-98) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 360.00 USD
to the supplier

Osteoprotegerin
HUABIO
catalog: EM1701-99

mouse monoclonal (9C2)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Osteoprotegerin on K562 cell lysate using anti-Osteoprotegerin antibody at 1/500 dilution.

Immunocytochemistry analysis of 293 cells labeling Osteoprotegerin with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Osteoprotegerin antibody (EM1701-99) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-99) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier

Human Osteoprotegerin
HUABIO
catalog: HA725067

domestic rabbit monoclonal (PSH13-31)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Osteoprotegerin matched pair antibodies Capture: HA725067, Human Osteoprotegerin Rabbit mAb [PSH13-31] Detector: HA725068, Human Osteoprotegerin Rabbit mAb [PSH13-32] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725067) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Osteoprotegerin protein (HA210977) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725068, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Osteoprotegerin in human serum samples. Capture: HA725067, Human Osteoprotegerin Rabbit mAb [PSH13-31] Detector: HA725068, Human Osteoprotegerin Rabbit mAb [PSH13-32] The concentrations of Osteoprotegerin were measured in duplicates, interpolated from the Osteoprotegerin standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteoprotegerin concentration was determined to be 641 pg/ml in human serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Osteoprotegerin
HUABIO
catalog: HA725068

domestic rabbit monoclonal (PSH13-32)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Osteoprotegerin matched pair antibodies Capture: HA725067, Human Osteoprotegerin Rabbit mAb [PSH13-31] Detector: HA725068, Human Osteoprotegerin Rabbit mAb [PSH13-32] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725067) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Osteoprotegerin protein (HA210977) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725068, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Osteoprotegerin in human serum samples. Capture: HA725067, Human Osteoprotegerin Rabbit mAb [PSH13-31] Detector: HA725068, Human Osteoprotegerin Rabbit mAb [PSH13-32] The concentrations of Osteoprotegerin were measured in duplicates, interpolated from the Osteoprotegerin standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteoprotegerin concentration was determined to be 641 pg/ml in human serum.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Osteoprotegerin
HUABIO
catalog: HA725069B

domestic rabbit monoclonal (PSH13-32)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Osteoprotegerin matched pair antibodies Capture: HA725067, Human Osteoprotegerin Rabbit mAb [PSH13-31] Detector: HA725068, Human Osteoprotegerin Rabbit mAb [PSH13-32] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725067) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Osteoprotegerin protein (HA210977) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725068, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Osteoprotegerin in human serum samples. Capture: HA725067, Human Osteoprotegerin Rabbit mAb [PSH13-31] Detector: HA725068, Human Osteoprotegerin Rabbit mAb [PSH13-32] The concentrations of Osteoprotegerin were measured in duplicates, interpolated from the Osteoprotegerin standard curve and corrected for sample dilution. Undiluted samples are human serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Osteoprotegerin concentration was determined to be 641 pg/ml in human serum.
quantity: 100μl
price: 409.00 USD
to the supplier

Osteoprotegerin
HUABIO
catalog: R1608-4

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Osteoprotegerin on different lysates with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/1,000 dilution. Lane 1: 293 cell lysate Lane 2: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 55 kDa Exposure time: 1 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1608-4) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of K-562 cells labeling Osteoprotegerin with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunocytochemistry analysis of Neuro-2a cells labeling Osteoprotegerin with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Osteoprotegerin antibody (R1608-4) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier