CD73
HUABIO
catalog: ET1703-41
domestic rabbit monoclonal (JM11-40)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD73 on different lysates with Rabbit anti-CD73 antibody (ET1703-41) at 1/2,000 dilution. Lane 1: HT-29 cell lysate Lane 2: U-87 MG cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-41) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD73 on different lysates with Rabbit anti-CD73 antibody (ET1703-41) at 1/2,000 dilution. Lane 1: HT-29 cell lysate Lane 2: HepG2 cell lysate Lane 3: A375 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 5 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-41) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-CD73 antibody (ET1703-41) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-41) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
CD73
HUABIO
catalog: HA601004
mouse monoclonal (A6G2)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD73 on different lysates with Mouse anti-CD73 antibody (HA601004) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: Raji cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601004) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-CD73 antibody (HA601004) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601004) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded U-87 MG xenograft tissue with Mouse anti-CD73 antibody (HA601004) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601004) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
CD73
HUABIO
catalog: HA601010
mouse monoclonal (A6F7)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD73 on different lysates with Mouse anti-CD73 antibody (HA601010) at 1/2,000 dilution. Lane 1: 4T1 cell lysate Lane 2: RAW264.7 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601010) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD73 on U-87 MG cell lysates with Mouse anti-CD73 antibody (HA601010) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 15 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601010) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-CD73 antibody (HA601010) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601010) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
CD73
HUABIO
catalog: HA601011
mouse monoclonal (A6F9)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD73 on different lysates with Mouse anti-CD73 antibody (HA601011) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: A375 cell lysate Lane 3: Raji cell lysate (negative) Lane 4: 4T1 cell lysate Lane 5: RAW264.7 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: Lane 1: 40 seconds; Lane 2-5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601011) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-CD73 antibody (HA601011) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601011) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded U-87 MG xenograft tissue with Mouse anti-CD73 antibody (HA601011) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601011) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
CD73
HUABIO
catalog: HA601012
mouse monoclonal (A6G1)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD73 on different lysates with Mouse anti-CD73 antibody (HA601012) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: Raji cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601012) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-CD73 antibody (HA601012) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601012) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded U-87 MG xenograft tissue with Mouse anti-CD73 antibody (HA601012) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601012) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
CD73
HUABIO
catalog: HA601299
mouse monoclonal (A6G2-R)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD73 on different lysates with Mouse anti-CD73 antibody (HA601299) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: Raji cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601299) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-CD73 antibody (HA601299) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601299) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded U-87 MG xenograft tissue with Mouse anti-CD73 antibody (HA601299) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601299) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
CD73
HUABIO
catalog: HA610172
mouse monoclonal (A6G2-R)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD73 on different lysates with Mouse anti-CD73 antibody (HA610172) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: Raji cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610172) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-CD73 antibody (HA610172) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610172) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded U-87 MG xenograft tissue with Mouse anti-CD73 antibody (HA610172) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610172) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier
Human CD73
HUABIO
catalog: HA722957
domestic rabbit monoclonal (PSH08-15)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD73 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722957) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant Human CD73 protein (HA210915) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA722957, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD73 in A431 extract samples based on a 1,000 µg/ml extract load. The concentrations of CD73 were measured in duplicates, interpolated from the CD73 standard curve and corrected for sample dilution. Undiluted samples are A431 extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD73 concentration was determined to be 6,201 pg/ml in A431 extract.

Interpolated concentrations of native CD73 in U-87 MG and Hela extract samples based on a 1,000 µg/ml extract load. The concentrations of CD73 were measured in duplicates, interpolated from the CD73 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG extract 13% and Hela extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD73 concentration was determined to be 70,356 pg/ml in U-87 MG extract and undetectable in Hela extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD73
HUABIO
catalog: HA722958
domestic rabbit monoclonal (PSH08-16)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human CD73 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722957) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant Human CD73 protein (HA210915) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA722957, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD73 in A431 extract samples based on a 1,000 µg/ml extract load. The concentrations of CD73 were measured in duplicates, interpolated from the CD73 standard curve and corrected for sample dilution. Undiluted samples are A431 extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD73 concentration was determined to be 6,201 pg/ml in A431 extract.

Interpolated concentrations of native CD73 in U-87 MG and Hela extract samples based on a 1,000 µg/ml extract load. The concentrations of CD73 were measured in duplicates, interpolated from the CD73 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG extract 13% and Hela extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD73 concentration was determined to be 70,356 pg/ml in U-87 MG extract and undetectable in Hela extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Human CD73
HUABIO
catalog: HA722959B
domestic rabbit monoclonal (PSH08-16)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human CD73 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722957) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant Human CD73 protein (HA210915) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA722957, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native CD73 in A431 extract samples based on a 1,000 µg/ml extract load. The concentrations of CD73 were measured in duplicates, interpolated from the CD73 standard curve and corrected for sample dilution. Undiluted samples are A431 extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD73 concentration was determined to be 6,201 pg/ml in A431 extract.

Interpolated concentrations of native CD73 in U-87 MG and Hela extract samples based on a 1,000 µg/ml extract load. The concentrations of CD73 were measured in duplicates, interpolated from the CD73 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG extract 13% and Hela extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD73 concentration was determined to be 70,356 pg/ml in U-87 MG extract and undetectable in Hela extract.
quantity: 100μl
price: 409.00 USD
to the supplier
CD73
HUABIO
catalog: HA723119
domestic rabbit monoclonal (PSH09-57)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD73 on different lysates with Rabbit anti-CD73 antibody (HA723119) at 1/2,000 dilution. Lane 1: HT-29 cell lysate Lane 2: U-87 MG cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: Lane 1: 3 minutes; Lane 2: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723119) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HT-29 (positive) and HeLa (low expression) labeling CD73 with Rabbit anti-CD73 antibody (HA723119) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD73 antibody (HA723119) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HT-29 cells labeling CD73. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723119, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/5,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
CD73
HUABIO
catalog: HA751304
domestic rabbit monoclonal (PSH09-57)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of CD73 on different lysates with Rabbit anti-CD73 antibody (HA751304) at 1/2,000 dilution. Lane 1: HT-29 cell lysate Lane 2: U-87 MG cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: Lane 1: 3 minutes; Lane 2: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751304) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HT-29 (positive) and HeLa (low expression) labeling CD73 with Rabbit anti-CD73 antibody (HA751304) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD73 antibody (HA751304) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HT-29 cells labeling CD73. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751304, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/5,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier
CD73
HUABIO
catalog: R1107-6
domestic rabbit polyclonal
reactivity: human, mouse
application: western blot, flow cytometry

Western blot analysis of CD73 on different cell lysates using anti-CD73 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: HT-29

ICC staining CD73 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining CD73 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier