Neuropeptide Y
HUABIO
catalog: HA723856
domestic rabbit monoclonal (PSH16-52)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA723856) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723856) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
Neuropeptide Y
HUABIO
catalog: HA723884
domestic rabbit monoclonal (PSH16-88)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of Human NPY matched pair antibodies Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723885, Neuropeptide Y Rabbit mAb [PSH16-89] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723884) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropeptide Y protein (HA211303) starting from 750 pg/ml to 0 pg/ml and detect antibody (HA723885, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human NPY matched pair antibodies Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723886, Neuropeptide Y Rabbit mAb [PSH16-90] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723884) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropeptide Y protein (HA211303) starting from 750 pg/ml to 0 pg/ml and detect antibody (HA723886, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NPY in mouse hippocampus and rat hippocampus tissue extract samples based on a 1000 µg/ml extract load. Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723885, Neuropeptide Y Rabbit mAb [PSH16-89] Interpolated concentration of native NPY was measured in duplicate at different sample concentrations and interpolated from the NPY standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NPY concentration was determined to be 1,192 pg/mL in mouse hippocampus and 565 pg/mL in rat hippocampus tissue extract.
quantity: 100μl
price: 649.00 USD
to the supplier
Neuropeptide Y
HUABIO
catalog: HA723885
domestic rabbit monoclonal (PSH16-89)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of Human NPY matched pair antibodies Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723885, Neuropeptide Y Rabbit mAb [PSH16-89] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723884) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropeptide Y protein (HA211303) starting from 750 pg/ml to 0 pg/ml and detect antibody (HA723885, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NPY in mouse hippocampus and rat hippocampus tissue extract samples based on a 1000 µg/ml extract load. Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723885, Neuropeptide Y Rabbit mAb [PSH16-89] Interpolated concentration of native NPY was measured in duplicate at different sample concentrations and interpolated from the NPY standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NPY concentration was determined to be 1,192 pg/mL in mouse hippocampus and 565 pg/mL in rat hippocampus tissue extract.

Interpolated concentrations of native NPY in human SH-SY5Y cell supernatant treated or untreated with TPA for 24 hours. Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723885, Neuropeptide Y Rabbit mAb [PSH16-89] Interpolated concentration of native NPY was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NPY concentration was determined to be 735 pg/mL in neat SH-SY5Y treated supernatant and undetectable in untreated SH-SY5Y supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Neuropeptide Y
HUABIO
catalog: HA723885B
domestic rabbit monoclonal (PSH16-89)
reactivity: human, mouse, rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human NPY matched pair antibodies Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723885, Neuropeptide Y Rabbit mAb [PSH16-89] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723884) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropeptide Y protein (HA211303) starting from 750 pg/ml to 0 pg/ml and detect antibody (HA723885, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NPY in mouse hippocampus and rat hippocampus tissue extract samples based on a 1000 µg/ml extract load. Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723885, Neuropeptide Y Rabbit mAb [PSH16-89] Interpolated concentration of native NPY was measured in duplicate at different sample concentrations and interpolated from the NPY standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NPY concentration was determined to be 1,192 pg/mL in mouse hippocampus and 565 pg/mL in rat hippocampus tissue extract.

Interpolated concentrations of native NPY in human SH-SY5Y cell supernatant treated or untreated with TPA for 24 hours. Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723885, Neuropeptide Y Rabbit mAb [PSH16-89] Interpolated concentration of native NPY was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NPY concentration was determined to be 735 pg/mL in neat SH-SY5Y treated supernatant and undetectable in untreated SH-SY5Y supernatant.
quantity: 100μl
price: 385.00 USD
to the supplier
Neuropeptide Y
HUABIO
catalog: HA723886
domestic rabbit monoclonal (PSH16-90)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of Human NPY matched pair antibodies Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723886, Neuropeptide Y Rabbit mAb [PSH16-90] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723884) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropeptide Y protein (HA211303) starting from 750 pg/ml to 0 pg/ml and detect antibody (HA723886, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NPY in mouse hippocampus and rat hippocampus tissue extract samples based on a 1000 µg/ml extract load. Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723886, Neuropeptide Y Rabbit mAb [PSH16-90] Interpolated concentration of native NPY was measured in duplicate at different sample concentrations and interpolated from the NPY standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NPY concentration was determined to be 1,226 pg/mL in mouse hippocampus and 514 pg/mL in rat hippocampus tissue extract.

Interpolated concentrations of native NPY in human SH-SY5Y cell supernatant treated or untreated with TPA for 24 hours. Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723886, Neuropeptide Y Rabbit mAb [PSH16-90] Interpolated concentration of native NPY was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NPY concentration was determined to be 583 pg/mL in neat SH-SY5Y treated supernatant and undetectable in untreated SH-SY5Y supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Neuropeptide Y
HUABIO
catalog: HA723886B
domestic rabbit monoclonal (PSH16-90)
reactivity: human, mouse, rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human NPY matched pair antibodies Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723886, Neuropeptide Y Rabbit mAb [PSH16-90] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723884) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neuropeptide Y protein (HA211303) starting from 750 pg/ml to 0 pg/ml and detect antibody (HA723886, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native NPY in mouse hippocampus and rat hippocampus tissue extract samples based on a 1000 µg/ml extract load. Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723886, Neuropeptide Y Rabbit mAb [PSH16-90] Interpolated concentration of native NPY was measured in duplicate at different sample concentrations and interpolated from the NPY standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NPY concentration was determined to be 1,226 pg/mL in mouse hippocampus and 514 pg/mL in rat hippocampus tissue extract.

Interpolated concentrations of native NPY in human SH-SY5Y cell supernatant treated or untreated with TPA for 24 hours. Capture: HA723884, Neuropeptide Y Rabbit mAb [PSH16-88] Detector: HA723886, Neuropeptide Y Rabbit mAb [PSH16-90] Interpolated concentration of native NPY was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NPY concentration was determined to be 583 pg/mL in neat SH-SY5Y treated supernatant and undetectable in untreated SH-SY5Y supernatant.
quantity: 100μl
price: 385.00 USD
to the supplier
Neuropeptide Y
HUABIO
catalog: HA751601
domestic rabbit monoclonal (PSH16-52)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue with Rabbit anti-Neuropeptide Y antibody (HA751601) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751601) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier