domestic rabbit monoclonal (PS01-10)
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Phospho-eNOS (S1177) on different lysates with Rabbit anti-Phospho-eNOS (S1177) antibody (HA721188) at 1/1,000 dilution. Lane 1: HAEC cell lysate (30 µg/Lane) Lane 2: HAEC serum starved then treated with 100nM Insulin for 1 hour cell lysate (30 µg/Lane) Predicted band size: 133 kDa Observed band size: 160 kDa Exposure time: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721188) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Phospho-eNOS (S1177) antibody (HA721188) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721188) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-96)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of eNOS on different lysates with Rabbit anti-eNOS antibody (HA723892) at 1/5,000 dilution. Lane 1: EA.hy926 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Lane 3: bEND.3 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse placenta tissue lysate (30 µg/Lane) Lane 6: Mouse kidney tissue lysate (30 µg/Lane) Lane 7: Rat placenta tissue lysate (30 µg/Lane) Lane 8: Rat kidney tissue lysate (30 µg/Lane) Predicted band size: 133 kDa Observed band size: 133 kDa Exposure time: Lane 1-2: 10 seconds; Lane 3-8: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723892) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of EA.hy926 (positive) and HeLa (negative) labeling eNOS with Rabbit anti-eNOS antibody (HA723892) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eNOS antibody (HA723892) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-eNOS antibody (HA723892) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723892) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PS01-10)
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat, dogs
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Phospho-eNOS (S1177) on different lysates with Rabbit anti-Phospho-eNOS (S1177) antibody (HA750522) at 1/1,000 dilution. Lane 1: HAEC cell lysate (30 µg/Lane) Lane 2: HAEC serum starved then treated with 100nM Insulin for 1 hour cell lysate (30 µg/Lane) Predicted band size: 133 kDa Observed band size: 160 kDa Exposure time: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750522) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Phospho-eNOS (S1177) antibody (HA750522) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750522) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH16-96)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of eNOS on different lysates with Rabbit anti-eNOS antibody (HA751610) at 1/5,000 dilution. Lane 1: EA.hy926 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (negative) (20 µg/Lane) Lane 3: bEND.3 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse placenta tissue lysate (30 µg/Lane) Lane 6: Mouse kidney tissue lysate (30 µg/Lane) Lane 7: Rat placenta tissue lysate (30 µg/Lane) Lane 8: Rat kidney tissue lysate (30 µg/Lane) Predicted band size: 133 kDa Observed band size: 133 kDa Exposure time: Lane 1-2: 10 seconds; Lane 3-8: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751610) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of EA.hy926 (positive) and HeLa (negative) labeling eNOS with Rabbit anti-eNOS antibody (HA751610) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eNOS antibody (HA751610) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-eNOS antibody (HA751610) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751610) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- eNOS rabbit polyclonal antibody.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- eNOS rabbit polyclonal antibody.
Immunohistochemical analysis of paraffin-embedded human mammary gland tissue using anti-eNOS rabbit polyclonal antibody.
quantity: 100μl
price: 330 USD
to the supplier