domestic rabbit monoclonal (SI70-01)
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (ET1606-20) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: NCCIT cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 24 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-20) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (ET1606-20) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: CRC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 25 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (ET1606-20) at 1/2,000 dilution. Lane 1: A549 WT cell lysate Lane 2: A549 ATM KO cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 1 minute 10 seconds; ECL: Ori Supersensitive 4-20% SDS-PAGE gel. ET1606-20 was shown to specifically react with ATM in A549 WT cells. No band was observed when A549 ATM KO sample was tested. A549 WT and A549 ATM KO samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-20, 1/2,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (JM93-25)
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Phospho-ATM (S1981) on different lysates with Rabbit anti-Phospho-ATM (S1981) antibody (ET1705-50) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 1μM Camptothecin for 1 hour cell lysate Lane 3: HeLa treated with 1μM Camptothecin for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-50) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-ATM (S1981) on different lysates with Rabbit anti-Phospho-ATM (S1981) antibody (ET1705-50) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20μM Etoposide for 2 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HEK-293 cell lysate Lane 5: MDA-MB-231 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-50) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells treated with or without 1μM Camptothecin for 1 hour labeling Phospho-ATM (S1981) with Rabbit anti-Phospho-ATM (S1981) antibody (ET1705-50) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-ATM (S1981) antibody (ET1705-50) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 50μl
price: 205.00 USD
to the supplier
domestic rabbit monoclonal (SI70-01)
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (HA750096) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: NCCIT cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 24 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750096) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (HA750096) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: CRC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 25 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750096) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (HA750096) at 1/2,000 dilution. Lane 1: A549 WT cell lysate Lane 2: A549 ATM KO cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 1 minute 10 seconds; ECL: Ori Supersensitive 4-20% SDS-PAGE gel. ET1606-20 was shown to specifically react with ATM in A549 WT cells. No band was observed when A549 ATM KO sample was tested. A549 WT and A549 ATM KO samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-20, 1/2,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (JM93-25)
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Phospho-ATM (S1981) on different lysates with Rabbit anti-Phospho-ATM (S1981) antibody (HA750432) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 1μM Camptothecin for 1 hour cell lysate Lane 3: HeLa treated with 1μM Camptothecin for 1 hour cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750432) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-ATM (S1981) on different lysates with Rabbit anti-Phospho-ATM (S1981) antibody (HA750432) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20μM Etoposide for 2 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HEK-293 cell lysate Lane 5: MDA-MB-231 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750432) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells treated with or without 1μM Camptothecin for 1 hour labeling Phospho-ATM (S1981) with Rabbit anti-Phospho-ATM (S1981) antibody (HA750432) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-ATM (S1981) antibody (HA750432) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier