MMP-9
HUABIO
catalog: ET1704-69-50UL
domestic rabbit monoclonal (JA80-73)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MMP-9 on different lysates with Rabbit anti-MMP-9 antibody (ET1704-69) at 1/5,000 dilution. Lane 1: THP-1 whole cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 200nM TPA for 10 minutes whole cell lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 78 kDa Observed band size: 92 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-69) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MMP9 on different lysates with Rabbit anti-MMP9 antibody (ET1704-69) at 1/5,000 dilution. Lane 1: A549-si NT+TPA(80nM 24h) cell lysate, 15 µg/Lane Lane 2: A549-si MMP9#1+TPA(80nM 24h) cell lysate, 15 µg/Lane Lane 3: A549-si MMP9#2+TPA(80nM 24h) cell lysate, 15 µg/Lane Predicted band size: 78 kDa Observed band size: 78-92 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-69, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MMP-9 antibody (ET1704-69) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1704-69) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 50μl
price: 205.00 USD
to the supplier
Human MMP-9
HUABIO
catalog: HA722106
domestic rabbit monoclonal (PSH04-23)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human MMP9 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722106) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP9 protein (HA210698) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722107) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are mixed serum from twenty-one volunteers 0.5%. The mean MMP9 concentration was determined to be 397.2 ng/ml in mixed serum from twenty-one volunteers.

The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are human urine 100%. The mean MMP9 concentration was determined to be 1624.2 pg/ml in human urine.
quantity: 100μl
price: 649.00 USD
to the supplier
Human MMP-9
HUABIO
catalog: HA722107
domestic rabbit monoclonal (PSH04-24)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human MMP9 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722106) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP9 protein (HA210698) starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722107) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are mixed serum from twenty-one volunteers 0.5%. The mean MMP9 concentration was determined to be 397.2 ng/ml in mixed serum from twenty-one volunteers.

The concentrations of MMP9 were interpolated from the MMP9 standard curves and corrected for sample dilution. Undiluted samples are human urine 100%. The mean MMP9 concentration was determined to be 1624.2 pg/ml in human urine.
quantity: 100μl
price: 649.00 USD
to the supplier
Human MMP-9
HUABIO
catalog: HA722162
domestic rabbit monoclonal (PSH04-62)
reactivity: human
application: ELISA
quantity: 100μl
price: 649.00 USD
to the supplier
Human MMP-9
HUABIO
catalog: HA722163
domestic rabbit monoclonal (PSH04-63)
reactivity: human
application: ELISA
quantity: 100μl
price: 649.00 USD
to the supplier
Human MMP-9
HUABIO
catalog: HA722615B
domestic rabbit monoclonal (PSH04-24)
reactivity: human
conjugate: biotin
application: ELISA
quantity: 100μl
price: 409.00 USD
to the supplier
MMP-9
HUABIO
catalog: HA722665
domestic rabbit monoclonal (PSH06-33)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of MMP-9 on different lysates with Rabbit anti-MMP-9 antibody (HA722665) at 1/2,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 200nM TPA for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 78 kDa Observed band size: 78/92 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722665) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MMP-9 antibody (HA722665) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722665) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MMP-9 antibody (HA722665) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722665) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
MMP-9
HUABIO
catalog: HA750420
domestic rabbit monoclonal (JA80-73)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MMP-9 on different lysates with Rabbit anti-MMP-9 antibody (HA750420) at 1/5,000 dilution. Lane 1: THP-1 whole cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 200nM TPA for 10 minutes whole cell lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 78 kDa Observed band size: 92 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750420) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MMP9 on different lysates with Rabbit anti-MMP9 antibody (HA750420) at 1/5,000 dilution. Lane 1: A549-si NT+TPA(80nM 24h) cell lysate, 15 µg/Lane Lane 2: A549-si MMP9#1+TPA(80nM 24h) cell lysate, 15 µg/Lane Lane 3: A549-si MMP9#2+TPA(80nM 24h) cell lysate, 15 µg/Lane Predicted band size: 78 kDa Observed band size: 78-92 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1704-69, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MMP-9 antibody (HA750420) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750420) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
MMP-9
HUABIO
catalog: HA751070
domestic rabbit monoclonal (PSH06-33)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of MMP-9 on different lysates with Rabbit anti-MMP-9 antibody (HA751070) at 1/2,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: U-2 OS treated with 200nM TPA for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 78 kDa Observed band size: 78/92 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751070) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MMP-9 antibody (HA751070) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751070) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MMP-9 antibody (HA751070) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751070) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
MMP-9
HUABIO
catalog: IRS172RB
domestic rabbit monoclonal
reactivity: human

mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-MMP-9 antibody (IRS172RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 50T
price: 278.00 USD
to the supplier