domestic rabbit monoclonal (JE59-38)
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: immunohistochemistry - paraffin section
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-MMP-8 antibody (HA721686) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721686) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MMP-8 antibody (HA721686) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721686) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-MMP-8 antibody (HA721686) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721686) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-33)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human MMP8 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721978) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human MMP8 protein (HA210646) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA721979, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
The concentrations of MMP8 were measured in duplicates, interpolated from the MMP8 standard curves and corrected for sample dilution. The mean MMP8 concentration was determined to be 1043.74 pg/mL in A375 supernatant.
The concentrations of MMP8 were measured in duplicates, interpolated from the MMP8 standard curves and corrected for sample dilution. The mean MMP8 concentration was determined to be 24841.38 pg/mL in SK-Mel-28 supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-34)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human MMP8 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721978) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human MMP8 protein (HA210646) starting from 5000 pg/ml to 0 pg/ml and detect antibody (HA721979, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
The concentrations of MMP8 were measured in duplicates, interpolated from the MMP8 standard curves and corrected for sample dilution. The mean MMP8 concentration was determined to be 1043.74 pg/mL in A375 supernatant.
The concentrations of MMP8 were measured in duplicates, interpolated from the MMP8 standard curves and corrected for sample dilution. The mean MMP8 concentration was determined to be 24841.38 pg/mL in SK-Mel-28 supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-34)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA