MMP-7
HUABIO
catalog: HA500305
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MMP7 on A549 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500305, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane: A549 cell lysate

Western blot analysis of MMP7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500305, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HT-29 cell lysate Lane 2: A431 cell lysate

ICC staining of MMP-7 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500305, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier
Human MMP-7
HUABIO
catalog: HA722926
domestic rabbit monoclonal (PSH07-93)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human MMP-7 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722926) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP-7 protein (HA210989) starting from 20000 pg/ml to 0 pg/ml and detect antibody (HA722927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native total MMP7 in human cell culture supernatant samples. Interpolated concentration of native total MMP-7 was measured in duplicate at different sample concentrations and interpolated from the total MMP-7 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean total MMP-7 concentration was determined to be 454,056 pg/mL in HT-29 and 1287 pg/ml in A549 in cell culture supernatant, undetectable in LNCaP supernatant.

Interpolated concentrations of spiked Pro-MMP-7 in cell culture media samples. The concentrations of Pro-MMP-7 were measured in duplicates, interpolated from thel Pro-MMP-7 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human MMP-7
HUABIO
catalog: HA722927
domestic rabbit monoclonal (PSH07-94)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human MMP-7 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722926) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP-7 protein (HA210989) starting from 20000 pg/ml to 0 pg/ml and detect antibody (HA722927, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native total MMP7 in human cell culture supernatant samples. Interpolated concentration of native total MMP-7 was measured in duplicate at different sample concentrations and interpolated from the total MMP-7 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean total MMP-7 concentration was determined to be 454,056 pg/mL in HT-29 and 1287 pg/ml in A549 in cell culture supernatant, undetectable in LNCaP supernatant.

Interpolated concentrations of spiked Pro-MMP-7 in cell culture media samples. The concentrations of Pro-MMP-7 were measured in duplicates, interpolated from thel Pro-MMP-7 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human MMP-7
HUABIO
catalog: HA722928B
domestic rabbit monoclonal (PSH07-94)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human MMP-7 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722926) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP-7 protein (HA210989) starting from 20000 pg/ml to 0 pg/ml and detect antibody (HA722928B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native total MMP7 in human cell culture supernatant samples. Interpolated concentration of native total MMP-7 was measured in duplicate at different sample concentrations and interpolated from the total MMP-7 standard curves. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean total MMP-7 concentration was determined to be 454,056 pg/mL in HT-29 and 1287 pg/ml in A549 in cell culture supernatant, undetectable in LNCaP supernatant.

Interpolated concentrations of spiked Pro-MMP-7 in cell culture media samples. The concentrations of Pro-MMP-7 were measured in duplicates, interpolated from thel Pro-MMP-7 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
MMP-7
HUABIO
catalog: HA723043
domestic rabbit monoclonal (PSH08-91)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MMP-7 on different lysates with Rabbit anti-MMP-7 antibody (HA723043) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 27 kDa Exposure time: Lane 1: 3 minutes; Lane 2: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723043) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HT-29 cells labeling MMP-7 with Rabbit anti-MMP-7 antibody (HA723043) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MMP-7 antibody (HA723043) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HT-29 cells labeling MMP-7. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723043, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
MMP-7
HUABIO
catalog: HA751256
domestic rabbit monoclonal (PSH08-91)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MMP-7 on different lysates with Rabbit anti-MMP-7 antibody (HA751256) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: HT-29 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 27 kDa Exposure time: Lane 1: 3 minutes; Lane 2: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751256) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of HT-29 cells labeling MMP-7 with Rabbit anti-MMP-7 antibody (HA751256) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MMP-7 antibody (HA751256) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Flow cytometric analysis of HT-29 cells labeling MMP-7. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751256, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier