domestic rabbit monoclonal (PSH12-34)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of CXCL9 on different lysates with Rabbit anti-CXCL9 antibody (HA723608) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 17 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723608) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of THP-1 cells untreated / treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours labeling CXCL9 with Rabbit anti-CXCL9 antibody (HA723608) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL9 antibody (HA723608) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-34)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human CXCL9 matched pair antibodies Capture: HA725004, Human CXCL9 Rabbit mAb [PSH12-34] Detector: HA725005, Human CXCL9 Rabbit mAb [PSH12-35] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725004) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL9 protein (HA210774) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725005, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CXCL9 in THP-1 cell culture supernatant. Capture: HA725004, Human CXCL9 Rabbit mAb [PSH12-34] Detector: HA725005, Human CXCL9 Rabbit mAb [PSH12-35] THP-1 cells were stimulated with 200 ng/ml IFN-γ and 50ng/ml LPS or vehicle control and incubated for 24 hours. The concentrations of CXCL9 measured in duplicate and interpolated from the CXCL9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 841.6 pg/ml in IFN-γ and LPS stimulated THP-1 cell culture supernatant and undetectable in the unstimulated THP-1 control.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-35)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human CXCL9 matched pair antibodies Capture: HA725004, Human CXCL9 Rabbit mAb [PSH12-34] Detector: HA725005, Human CXCL9 Rabbit mAb [PSH12-35] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725004) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL9 protein (HA210774) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725005, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CXCL9 in THP-1 cell culture supernatant. Capture: HA725004, Human CXCL9 Rabbit mAb [PSH12-34] Detector: HA725005, Human CXCL9 Rabbit mAb [PSH12-35] THP-1 cells were stimulated with 200 ng/ml IFN-γ and 50ng/ml LPS or vehicle control and incubated for 24 hours. The concentrations of CXCL9 measured in duplicate and interpolated from the CXCL9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 841.6 pg/ml in IFN-γ and LPS stimulated THP-1 cell culture supernatant and undetectable in the unstimulated THP-1 control.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-35)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human CXCL9 matched pair antibodies Capture: HA725004, Human CXCL9 Rabbit mAb [PSH12-34] Detector: HA725005, Human CXCL9 Rabbit mAb [PSH12-35] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725004) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL9 protein (HA210774) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725005, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CXCL9 in THP-1 cell culture supernatant. Capture: HA725004, Human CXCL9 Rabbit mAb [PSH12-34] Detector: HA725005, Human CXCL9 Rabbit mAb [PSH12-35] THP-1 cells were stimulated with 200 ng/ml IFN-γ and 50ng/ml LPS or vehicle control and incubated for 24 hours. The concentrations of CXCL9 measured in duplicate and interpolated from the CXCL9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 841.6 pg/ml in IFN-γ and LPS stimulated THP-1 cell culture supernatant and undetectable in the unstimulated THP-1 control.
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-34)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of CXCL9 on different lysates with Rabbit anti-CXCL9 antibody (HA751512) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 17 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751512) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of THP-1 cells untreated / treated with 100ng/mL IFN-gamma for 24 hours then treated with 300ng/mL BFA for 20 hours labeling CXCL9 with Rabbit anti-CXCL9 antibody (HA751512) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CXCL9 antibody (HA751512) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier