MICA + MICB
HUABIO
catalog: ER1803-95
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (ER1803-95) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HL-60 cell lysate Lane 4: HUVEC cell lysate Lane 5: THP-1 cell lysate (negative) Lane 6: NIH:OVCAR-3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-95) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
Human MICB
HUABIO
catalog: HA723238
domestic rabbit monoclonal (PSH10-73)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human MICB matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723238) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MICB protein (HA210907) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723239, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MICB in T-47D and PC-3 extract samples based on a 1,000 µg/ml extract load. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are T-47D extract 100% and PC-3 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 5,368 pg/ml in T-47D extract and undetectable in PC-3 extract.

Interpolated concentrations of native MICB in MCF-7 and PC-3 cell culture supernatant. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are MCF-7 cell culture supernatant 100% and PC-3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 334.07 pg/ml in MCF-7 cell culture supernatant and undetectable in PC-3 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human MICB
HUABIO
catalog: HA723239
domestic rabbit monoclonal (PSH10-74)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human MICB matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723238) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MICB protein (HA210907) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723239, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MICB in T-47D and PC-3 extract samples based on a 1,000 µg/ml extract load. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are T-47D extract 100% and PC-3 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 5,368 pg/ml in T-47D extract and undetectable in PC-3 extract.

Interpolated concentrations of native MICB in MCF-7 and PC-3 cell culture supernatant. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are MCF-7 cell culture supernatant 100% and PC-3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 334.07 pg/ml in MCF-7 cell culture supernatant and undetectable in PC-3 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
Human MICB
HUABIO
catalog: HA723240B
domestic rabbit monoclonal (PSH10-74)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human MICB matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723238) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MICB protein (HA210907) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723240B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native MICB in T-47D and PC-3 extract samples based on a 1,000 µg/ml extract load. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are T-47D extract 100% and PC-3 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 5,368 pg/ml in T-47D extract and undetectable in PC-3 extract.

Interpolated concentrations of native MICB in MCF-7 and PC-3 cell culture supernatant. The concentrations of MICB were measured in duplicates, interpolated from the MICB standard curve and corrected for sample dilution. Undiluted samples are MCF-7 cell culture supernatant 100% and PC-3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MICB concentration was determined to be 334.07 pg/ml in MCF-7 cell culture supernatant and undetectable in PC-3 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier
MICA + MICB
HUABIO
catalog: HA723255
domestic rabbit monoclonal (PSH10-88)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA723255) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (25 µg/Lane) Lane 3: THP-1 cell lysate (negative) (25 µg/Lane) Lane 4: NIH:OVCAR-3 cell lysate (25 µg/Lane) Lane 5: PC-3 cell lysate (low expression) (25 µg/Lane) Lane 6: U-2 OS cell lysate (25 µg/Lane) Lane 7: HUVEC cell lysate (25 µg/Lane) Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723255) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA723255) at 1/2,000 dilution. Lane 1: Human MICA recombinant protein, 20ng/Lane Lane 2: Human MICB recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723255) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA + MICB. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA723255, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
MICA + MICB
HUABIO
catalog: HA751380
domestic rabbit monoclonal (PSH10-88)
reactivity: human
application: western blot, immunoprecipitation, flow cytometry

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA751380) at 1/5,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (25 µg/Lane) Lane 3: THP-1 cell lysate (negative) (25 µg/Lane) Lane 4: NIH:OVCAR-3 cell lysate (25 µg/Lane) Lane 5: PC-3 cell lysate (low expression) (25 µg/Lane) Lane 6: U-2 OS cell lysate (25 µg/Lane) Lane 7: HUVEC cell lysate (25 µg/Lane) Predicted band size: 43 kDa Observed band size: 60 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751380) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of MICA + MICB on different lysates with Rabbit anti-MICA + MICB antibody (HA751380) at 1/2,000 dilution. Lane 1: Human MICA recombinant protein, 20ng/Lane Lane 2: Human MICB recombinant protein, 20ng/Lane Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751380) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of THP-1 (left, negative) and HeLa (right, positive) cells labeling MICA + MICB. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA751380, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier