Tau
HUABIO
catalog: ET1603-2

domestic rabbit monoclonal (SZ03-03)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling Tau with Rabbit anti-Tau antibody (ET1603-2). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1603-2, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Tau with Rabbit anti-Tau antibody (ET1603-2). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1603-2, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Western blot analysis of Tau on mouse brain tissue lysates with Rabbit anti-Tau antibody (ET1603-2) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 50 kDa Exposure time: 3 minutes 49 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (T231)
HUABIO
catalog: ET1610-31

domestic rabbit monoclonal (SC58-08)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Phospho-Tau (T231) on different lysates with Rabbit anti-Phospho-Tau (T231) antibody (ET1610-31) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain treated with λpp for 1 hour tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-31) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (ET1610-31) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-31) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (ET1610-31) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-31) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S396)
HUABIO
catalog: ET1611-68-50UL

domestic rabbit monoclonal (SN62-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Phospho-Tau (S396) on SHSY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-Tau(S396) on SHSY5Y cell lysates. Lane 1: SHSY5Y cells, whole cell lysate, 10ug/lane Lane 2: SHSY5Y cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Tau(S396) antibody (ET1611-68) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 79 kDa Observed band size: 70/130 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 34 seconds

ICC staining of Phospho-Tau (S396) in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-68, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 50μl
price: 205.00 USD
to the supplier

Tau
HUABIO
catalog: ET1612-44

domestic rabbit monoclonal (SD205-09)
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (ET1612-44) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse hippocampus tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat hippocampus tissue lysate (20 µg/Lane) Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-44) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.

Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (T231)
HUABIO
catalog: HA721569

domestic rabbit monoclonal (PSH01-05)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Tau (T231) on different lysates with Rabbit anti-Phospho-Tau (T231) antibody (HA721569) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 µg/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721569) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA721569) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721569) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA721569) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721569) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (T231)
HUABIO
catalog: HA721570

domestic rabbit monoclonal (PSH01-04)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Tau (T231) on different lysates with Rabbit anti-Phospho-Tau (T231) antibody (HA721570) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 µg/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721570) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA721570) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA721570) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S404)
HUABIO
catalog: HA721665

domestic rabbit monoclonal (JE66-82)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry

Western blot analysis of Phospho-Tau (S404) on different lysates with Rabbit anti-Phospho-Tau (S404) antibody (HA721665) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 30 µg/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721665) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (S404) antibody (HA721665) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721665) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S202)
HUABIO
catalog: HA721666

domestic rabbit monoclonal (JE66-81)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Tau (S202) on different lysates with Rabbit anti-Phospho-Tau (S202) antibody (HA721666) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse brain treated with λpp for 1 hour tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721666) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S199)
HUABIO
catalog: HA721667

domestic rabbit monoclonal (JE66-80)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Tau (S199) on different lysates with Rabbit anti-Phospho-Tau (S199) antibody (HA721667) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain treated with λpp for 1 hour tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721667) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S198)
HUABIO
catalog: HA721668

domestic rabbit monoclonal (JE66-79)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Tau (S198) on different lysates with Rabbit anti-Phospho-Tau (S198) antibody (HA721668) at 1/2,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse hippocampus treated with λpp for 1 hour tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721668) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S214)
HUABIO
catalog: HA721797

domestic rabbit monoclonal (JE66-83)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Tau (S214) on different lysates with Rabbit anti-Phospho-Tau (S214) antibody (HA721797) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721797) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S324)
HUABIO
catalog: HA721798

domestic rabbit monoclonal (JE30-73)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Tau (S324) on different lysates with Rabbit anti-Phospho-Tau (S324) antibody (HA721798) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 35-130 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721798) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S356)
HUABIO
catalog: HA721799

domestic rabbit monoclonal (JE46-86)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Tau (S356) on different lysates with Rabbit anti-Phospho-Tau (S356) antibody (HA721799) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour, then treated with λpp for 1 hour cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 50-250 kDa Exposure time: 3 minutes 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721799) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S199)
HUABIO
catalog: HA721800

domestic rabbit monoclonal (JE46-95)
reactivity: human, mouse, rat
application: western blot

Western blot analysis of Phospho-Tau (S199) on different lysates with Rabbit anti-Phospho-Tau (S199) antibody (HA721800) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Human brain tissue lysate, the membrane treated with λpp for 1 hour Lane 5: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lane 6: Rat brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 35-70 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721800) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (S404)
HUABIO
catalog: HA721801

domestic rabbit monoclonal (JE47-88)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Tau (S404) on different lysates with Rabbit anti-Phospho-Tau (S404) antibody (HA721801) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 30 µg/Lane. Predicted band size: 79 kDa Observed band size: 50-70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721801) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (S404) antibody (HA721801) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721801) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (T181)
HUABIO
catalog: HA722271

domestic rabbit monoclonal (PSH05-42)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Tau (T181) on different lysates with Rabbit anti-Phospho-Tau (T181) antibody (HA722271) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 40 µg/Lane. Predicted band size: 79 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722271) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T181) antibody (HA722271) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722271) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Phospho-Tau (T217)
HUABIO
catalog: HA723091

domestic rabbit monoclonal (PSH09-33)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Phospho-Tau (T217) on different lysates with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/2,000 dilution. Lane 1: 293T transfected with Tau cell lysate Lane 2: 293T transfected with Tau (mutated T217A) cell lysate (negative) Lysates/proteins at 15 µg/Lane. Predicted band size: 79 kDa Observed band size: 55-75 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723091) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of 293T cells labeling Phospho-Tau (T217) with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/500 dilution. 293T cells, transfected with empty control (top, negative) / Tau (middle, positive) / Tau (mutated T217A) (bottom, negative) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Western blot analysis of Phospho-Tau (T217) on different lysates with Rabbit anti-Phospho-Tau (T217) antibody (HA723091) at 1/1,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 55-75 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723091) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Tau
HUABIO
catalog: HA723209

domestic rabbit monoclonal (PSH10-42)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723841, Phospho-Tau (T217) Rabbit mAb [PSH16-41] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723841) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies Capture: HA723843, Phospho-Tau (T217) Rabbit mAb [PSH16-43] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723843) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723209, Brain-derived tau/BD-tau Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Tau
HUABIO
catalog: HA723209B

domestic rabbit monoclonal (PSH10-42)
reactivity: human, mouse, rat
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723841, Phospho-Tau (T217) Rabbit mAb [PSH16-41] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723841) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies Capture: HA723843, Phospho-Tau (T217) Rabbit mAb [PSH16-43] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723843) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723209, Brain-derived tau/BD-tau Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Phospho-Tau (T217)
HUABIO
catalog: HA723841

domestic rabbit monoclonal (PSH16-41)
reactivity: human, mouse
application: ELISA

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723841, Phospho-Tau (T217) Rabbit mAb [PSH16-41] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723841) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of Tau (phospho T217) in 293T+Human Tau441 2N4R and 293T+OE-Vector Cell Extract samples based on a 1000 µg/ml extract load. 293T+Human Tau441 2N4R represented the overexpression of Tau (phospho T217) protein. 293T+OE-Vector represented the non-expression of Tau (phospho T217) protein. Capture: HA723841, Phospho-Tau (T217) Rabbit mAb [PSH16-41] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (phospho T217) concentration was determined to be 18 ug/mL in 293T+Human Tau441 2N4R cell extract. There was no detectable signal in 293T+OE-Vector cell extract.

Interpolated concentrations of Tau (phospho T217) in mouse brain tissue extract samples based on a 1000 µg/ml extract load. Capture: HA723841, Phospho-Tau (T217) Rabbit mAb [PSH16-41] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The mean Tau (phospho T217) concentration was determined to be 945,192 pg/mL in mouse brain tissue extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Tau (T217)
HUABIO
catalog: HA723842

domestic rabbit monoclonal (PSH16-42)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723842, Phospho-Tau (T217) Rabbit mAb [PSH16-42] Detector: HA723898, Phospho-Tau (T217) Rabbit mAb [PSH17-11] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723842) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA723898, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of Tau (phospho T217) in 293T+Human Tau441 2N4R and 293T+OE-Vector Cell Extract samples based on a 1000 µg/ml extract load. 293T+Human Tau441 2N4R represented the overexpression of Tau (phospho T217) protein. 293T+OE-Vector represented the non-expression of Tau (phospho T217) protein. Capture: HA723842, Phospho-Tau (T217) Rabbit mAb [PSH16-42] Detector: HA723898, Phospho-Tau (T217) Rabbit mAb [PSH17-11] Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (phospho T217) concentration was determined to be 19 ug/mL in 293T+Human Tau441 2N4R cell extract. There was no detectable signal in 293T+OE-Vector cell extract.

Coating Ab: HA723842, 5ug/mL Co-Incubation: 1:2 serially diluted human Tau (phospho T217) and Tau (Non-phospho T217) protein from HuaBio starting from a concentration of 50000 pg/ml and Detect Ab: (HA723209, biotin, 0.2 ug/ml) (30℃, 120 rpm, 60min). SA-HRP:1:20000(50ng/ml) (30°c, 120rpm, 30min).
quantity: 100μl
price: 649.00 USD
to the supplier

Non-Phospho-Tau (T217)
HUABIO
catalog: HA723843

domestic rabbit monoclonal (PSH16-43)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies Capture: HA723843, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-43] Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723843) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Non-p217 Tau in rat cerebellum, SH-SY5Y and MDA-MB-231 extract samples based on a 1000 µg/ml extract load. Capture: HA723843, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-43] Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42] Interpolated concentration of native Non-p217 Tau was measured in duplicate at different sample concentrations and interpolated from the Non-p217 Tau standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Non-p217 Tau concentration was determined to be 561,264 pg/mL in rat cerebellum and 66,440 pg/mL in SH-SY5Y cell extract, There was no detectable signal in MDA-MB-231 cell extract.

Interpolated concentrations of spiked Non-p217 Tau in cell culture media samples. Capture: HA723843, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-43] Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42] The concentrations of Non-p217 Tau were measured in duplicates, interpolated from the Non-p217 Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Non-Phospho-Tau (T217)
HUABIO
catalog: HA723844

domestic rabbit monoclonal (PSH16-44)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies Capture: HA723844, Phospho-Tau (T217) Rabbit mAb [PSH16-44] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723844) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Non-p217 Tau in rat cerebellum, SH-SY5Y and MDA-MB-231 extract samples based on a 1000 µg/ml extract load. Capture: HA723844, Phospho-Tau (T217) Rabbit mAb [PSH16-44] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Interpolated concentration of native Non-p217 Tau was measured in duplicate at different sample concentrations and interpolated from the Non-p217 Tau standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Non-p217 Tau concentration was determined to be 35,404 pg/mL in SH-SY5Y cell extract, There was no detectable signal in MDA-MB-231 cell extract.

Interpolated concentrations of spiked Non-p217 Tau in cell culture media samples. Capture: HA723844, Phospho-Tau (T217) Rabbit mAb [PSH16-44] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] The concentrations of Non-p217 Tau were measured in duplicates, interpolated from the Non-p217 Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Non-Phospho-Tau (T217)
HUABIO
catalog: HA723845

domestic rabbit monoclonal (PSH16-45)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies Capture: HA723844, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44] Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723844) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Non-p217 Tau in rat cerebellum, SH-SY5Y and MDA-MB-231 extract samples based on a 1000 µg/ml extract load. Capture: HA723844, Phospho-Tau (T217) Rabbit mAb [PSH16-44] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Interpolated concentration of native Non-p217 Tau was measured in duplicate at different sample concentrations and interpolated from the Non-p217 Tau standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Non-p217 Tau concentration was determined to be 35,404 pg/mL in SH-SY5Y cell extract, There was no detectable signal in MDA-MB-231 cell extract.

Interpolated concentrations of spiked Non-p217 Tau in cell culture media samples. Capture: HA723844, Phospho-Tau (T217) Rabbit mAb [PSH16-44] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] The concentrations of Non-p217 Tau were measured in duplicates, interpolated from the Non-p217 Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Non-Phospho-Tau (T217)
HUABIO
catalog: HA723846B

domestic rabbit monoclonal (PSH16-45)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Non-p217 Tau matched pair antibodies Capture: HA723844, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44] Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723844) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA210937) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Non-p217 Tau in rat cerebellum, SH-SY5Y and MDA-MB-231 extract samples based on a 1000 µg/ml extract load. Capture: HA723844, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44] Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42] Interpolated concentration of native Non-p217 Tau was measured in duplicate at different sample concentrations and interpolated from the Non-p217 Tau standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Non-p217 Tau concentration was determined to be 35,404 pg/mL in SH-SY5Y cell extract, There was no detectable signal in MDA-MB-231 cell extract.

Interpolated concentrations of spiked Non-p217 Tau in cell culture media samples. Capture: HA723844, Non-Phospho-Tau (T217) Rabbit mAb [PSH16-44] Detector: HA723209, Non-Phospho-Tau (T217) Rabbit mAb [PSH10-42] The concentrations of Non-p217 Tau were measured in duplicates, interpolated from the Non-p217 Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

Brain-derived tau/BD-tau
HUABIO
catalog: HA723847

domestic rabbit monoclonal (PSH16-46)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723209, Brain-derived tau/BD-tau Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723848, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Brain-derived Tau in rat cerebellum and SH-SY5Y extract samples based on a 1000 µg/ml extract load. Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723209, Brain-derived tau/BD-tau Rabbit mAb [PSH10-42] Interpolated concentration of native Brain-derived Tau was measured in duplicate at different sample concentrations and interpolated from the Brain-derived Tau standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Brain-derived Tau concentration was determined to be 386,318 pg/mL in rat cerebellum and 79,826 pg/mL in SH-SY5Y cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Brain-derived tau/BD-tau
HUABIO
catalog: HA723848

domestic rabbit monoclonal (PSH16-47)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723848, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Brain-derived Tau in SH-SY5Y and MDA-MB-231 cell extract samples based on a 1000 µg/ml extract load. Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] Interpolated concentration of native Brain-derived Tau was measured in duplicate at different sample concentrations and interpolated from the Brain-derived Tau standard curves. The mean Brain-derived Tau concentration was determined to be 40,370 pg/mL in SH-SY5Y cell extract. There was no detectable signal in MDA-MB-231 cell extract.

Interpolated concentrations of spiked Brain-derived Tau in cell culture media samples. Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] The concentrations of Brain-derived Tau were measured in duplicates, interpolated from the Brain-derived Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Brain-derived tau/BD-tau
HUABIO
catalog: HA723849B

domestic rabbit monoclonal (PSH16-47)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Brain-derived tau/BD-tau matched pair antibodies Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723847) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Brain-derived tau/BD-tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723848, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Brain-derived Tau in SH-SY5Y and MDA-MB-231 cell extract samples based on a 1000 µg/ml extract load. Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] Interpolated concentration of native Brain-derived Tau was measured in duplicate at different sample concentrations and interpolated from the Brain-derived Tau standard curves. The mean Brain-derived Tau concentration was determined to be 40,370 pg/mL in SH-SY5Y cell extract. There was no detectable signal in MDA-MB-231 cell extract.

Interpolated concentrations of spiked Brain-derived Tau in cell culture media samples. Capture: HA723847, Brain-derived tau/BD-tau Rabbit mAb [PSH16-46] Detector: HA723848, Brain-derived tau/BD-tau Rabbit mAb [PSH10-47] The concentrations of Brain-derived Tau were measured in duplicates, interpolated from the Brain-derived Tau standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier

Tau
HUABIO
catalog: HA723850

domestic rabbit monoclonal (PSH16-48)
reactivity: human, mouse, rat
application: ELISA

Sandwich ELISA analysis of human Tau matched pair antibodies Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723209, Tau Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of human Tau matched pair antibodies Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723209, Tau Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA211246) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Tau in SH-SY5Y and mouse cerebellum extract samples based on a 1000 µg/ml extract load. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723209, Tau Rabbit mAb [PSH10-42] Interpolated concentration of native Tau was measured in duplicate at different sample concentrations and interpolated from the Tau standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau concentration was determined to be 14,861 pg/mL in SH-SY5Y cell extract and 3,257 pg/mL in mouse cerebellum tissue extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Tau
HUABIO
catalog: HA723851

domestic rabbit monoclonal (PSH16-49)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Tau matched pair antibodies Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723851, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Tau (4R Isoforms) in SH-SY5Y extract samples based on a 1000 µg/ml extract load. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] Interpolated concentration of native Tau (4R Isoforms) was measured in duplicate at different sample concentrations and interpolated from the Tau (4R Isoforms) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (4R Isoforms) concentration was determined to be 41,927 pg/mL in SH-SY5Y cell extract.

Interpolated concentrations of native Tau (4R Isoforms) in human cell culture supernatant samples. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] Interpolated concentration of native Tau (4R Isoforms) was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (4R Isoforms) concentration was determined to be 2,372 pg/mL in SH-SY5Y cell supernatant. There was no detectable signal in MDA-MB-231 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Tau
HUABIO
catalog: HA723852B

domestic rabbit monoclonal (PSH16-49)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human Tau matched pair antibodies Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723850) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tau protein (HA210937) starting from 10000 pg/ml to 0 pg/ml and detect antibody (HA723851, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Tau (4R Isoforms) in SH-SY5Y extract samples based on a 1000 µg/ml extract load. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] Interpolated concentration of native Tau (4R Isoforms) was measured in duplicate at different sample concentrations and interpolated from the Tau (4R Isoforms) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (4R Isoforms) concentration was determined to be 41,927 pg/mL in SH-SY5Y cell extract.

Interpolated concentrations of native Tau (4R Isoforms) in human cell culture supernatant samples. Capture: HA723850, Tau Rabbit mAb [PSH16-48] Detector: HA723851, Tau Rabbit mAb [PSH16-49] Interpolated concentration of native Tau (4R Isoforms) was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (4R Isoforms) concentration was determined to be 2,372 pg/mL in SH-SY5Y cell supernatant. There was no detectable signal in MDA-MB-231 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

Phospho-Tau (T217)
HUABIO
catalog: HA723877

domestic rabbit monoclonal (PSH16-75)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723877, Phospho-Tau (T217) Rabbit mAb [PSH16-75] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723877) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 15000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of Tau (phospho T217) in 293T+OE-human Tau441 2N4R and 293T+OE-Vector extract samples based on a 1000 µg/ml extract load. 293T+OE-human Tau441 2N4R represented the overexpression of Tau (phospho T217) protein. 293T+OE-Vector represented the non-expression of Tau (phospho T217) protein. Capture: HA723877, Phospho-Tau (T217) Rabbit mAb [PSH16-75] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (phospho T217) concentration was determined to be 258 ug/mL in 293T+OE-human Tau441 2N4R cell extract. There was no detectable signal in 293T+OE-Vector cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Tau
HUABIO
catalog: HA723898

domestic rabbit monoclonal (PSH17-11)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723842, Phospho-Tau (T217) Rabbit mAb [PSH16-42] Detector: HA723898, Phospho-Tau (T217) Rabbit mAb [PSH17-11] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723842) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA723898, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of Tau (phospho T217) in 293T+Human Tau441 2N4R and 293T+OE-Vector Cell Extract samples based on a 1000 µg/ml extract load. 293T+Human Tau441 2N4R represented the overexpression of Tau (phospho T217) protein. 293T+OE-Vector represented the non-expression of Tau (phospho T217) protein. Capture: HA723842, Phospho-Tau (T217) Rabbit mAb [PSH16-42] Detector: HA723898, Phospho-Tau (T217) Rabbit mAb [PSH17-11] Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (phospho T217) concentration was determined to be 19 ug/mL in 293T+Human Tau441 2N4R cell extract. There was no detectable signal in 293T+OE-Vector cell extract.

Coating Ab: HA723842, 5ug/mL Co-Incubation: 1:2 serially diluted human Tau (phospho T217) and Tau (Non-phospho T217) protein from HuaBio starting from a concentration of 50000 pg/ml and Detect Ab: (HA723209, biotin, 0.2 ug/ml) (30℃, 120 rpm, 60min). SA-HRP:1:20000(50ng/ml) (30°c, 120rpm, 30min).
quantity: 100μl
price: 649.00 USD
to the supplier

Tau
HUABIO
catalog: HA723898B

domestic rabbit monoclonal (PSH17-11)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723842, Phospho-Tau (T217) Rabbit mAb [PSH16-42] Detector: HA723898, Phospho-Tau (T217) Rabbit mAb [PSH17-11] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723842) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50000 pg/ml to 0 pg/ml and detect antibody (HA723898, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of Tau (phospho T217) in 293T+Human Tau441 2N4R and 293T+OE-Vector Cell Extract samples based on a 1000 µg/ml extract load. 293T+Human Tau441 2N4R represented the overexpression of Tau (phospho T217) protein. 293T+OE-Vector represented the non-expression of Tau (phospho T217) protein. Capture: HA723842, Phospho-Tau (T217) Rabbit mAb [PSH16-42] Detector: HA723898, Phospho-Tau (T217) Rabbit mAb [PSH17-11] Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (phospho T217) concentration was determined to be 19 ug/mL in 293T+Human Tau441 2N4R cell extract. There was no detectable signal in 293T+OE-Vector cell extract.

Coating Ab: HA723842, 5ug/mL Co-Incubation: 1:2 serially diluted human Tau (phospho T217) and Tau (Non-phospho T217) protein from HuaBio starting from a concentration of 50000 pg/ml and Detect Ab: (HA723209, biotin, 0.2 ug/ml) (30℃, 120 rpm, 60min). SA-HRP:1:20000(50ng/ml) (30°c, 120rpm, 30min).
quantity: 100μl
price: 409.00 USD
to the supplier

Tau
HUABIO
catalog: HA723913

domestic rabbit monoclonal (PSH17-26)
reactivity: human
application: ELISA

Indirect ELISA antigen dose-response curve using HA723913 at 1000-0 ng/mL. Antigen Human Tau protein at concentration of 1 ug/mL. HRP-conjugated Goat Anti-Rabbit IgG H+L at 1/5000 dilution was used as the secondary antibody.
quantity: 100μl
price: 649.00 USD
to the supplier

Tau
HUABIO
catalog: HA723914

domestic rabbit monoclonal (PSH17-27)
reactivity: human
application: ELISA

Indirect ELISA antigen dose-response curve using HA723913 at 1000-0 ng/mL. Antigen Human Tau protein at concentration of 1 ug/mL. HRP-conjugated Goat Anti-Rabbit IgG H+L at 1/5000 dilution was used as the secondary antibody.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Tau (T217)
HUABIO
catalog: HA723922

domestic rabbit monoclonal (PSH17-41)
reactivity: human, mouse
application: ELISA

Sandwich ELISA analysis of Human Tau (phospho T217) matched pair antibodies Capture: HA723922, Phospho-Tau (T217) Rabbit mAb [PSH17-41] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723922) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Phospho-Tau (T217) protein (HA211033) starting from 50,000 pg/ml to 0 pg/ml and detect antibody (HA723209, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of Tau (phospho T217) in 293T+Human Tau441 2N4R and 293T+OE-Vector Cell Extract samples based on a 1000 µg/ml extract load. 293T+Human Tau441 2N4R represented the overexpression of Tau (phospho T217) protein. 293T+OE-Vector represented the non-expression of Tau (phospho T217) protein. Capture: HA723922, Phospho-Tau (T217) Rabbit mAb [PSH17-41] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Tau (phospho T217) concentration was determined to be 18 ug/mL in 293T+Human Tau441 2N4R cell extract. There was no detectable signal in 293T+OE-Vector cell extract.

Interpolated concentrations of Tau (phospho T217) in mouse brain tissue extract samples based on a 1,000 µg/ml extract load. Capture: HA723922, Phospho-Tau (T217) Rabbit mAb [PSH17-41] Detector: HA723209, Phospho-Tau (T217) Rabbit mAb [PSH10-42] Interpolated concentration of Tau (phospho T217) was measured in duplicate at different sample concentrations and interpolated from the Tau (phospho T217) standard curves. The mean Tau (phospho T217) concentration was determined to be 567,31 pg/mL in mouse brain tissue extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Tau (T231)
HUABIO
catalog: HA750210

domestic rabbit monoclonal (SC58-08)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Phospho-Tau (T231) on different lysates with Rabbit anti-Phospho-Tau (T231) antibody (HA750210) at 1/2,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Mouse brain treated with λpp for 1 hour tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750210) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA750210) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA750210) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750210) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Tau (S396)
HUABIO
catalog: HA750268

domestic rabbit monoclonal (SN62-09)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Phospho-Tau (S396) on SHSY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750268, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Phospho-Tau(S396) on SHSY5Y cell lysates. Lane 1: SHSY5Y cells, whole cell lysate, 10ug/lane Lane 2: SHSY5Y cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-Tau(S396) antibody (HA750268) at 1/500 dilution. Anti-GAPDH antibody (ET1601-4) at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 79 kDa Observed band size: 70/130 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 34 seconds

ICC staining of Phospho-Tau (S396) in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750268, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Tau (T231)
HUABIO
catalog: HA750679

domestic rabbit monoclonal (PSH01-05)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Tau (T231) on different lysates with Rabbit anti-Phospho-Tau (T231) antibody (HA750679) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 µg/Lane. Predicted band size: 46 kDa Observed band size: 35-70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750679) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA750679) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750679) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA750679) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750679) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Tau (T231)
HUABIO
catalog: HA750680

domestic rabbit monoclonal (PSH01-04)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Tau (T231) on different lysates with Rabbit anti-Phospho-Tau (T231) antibody (HA750680) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 50 µg/Lane. Predicted band size: 46 kDa Observed band size: 35-100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750680) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA750680) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750680) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T231) antibody (HA750680) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750680) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Tau (T181)
HUABIO
catalog: HA751001

domestic rabbit monoclonal (PSH05-42)
reactivity: human, mouse
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Phospho-Tau (T181) on different lysates with Rabbit anti-Phospho-Tau (T181) antibody (HA751001) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Human brain tissue lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 40 µg/Lane. Predicted band size: 79 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751001) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-Tau (T181) antibody (HA751001) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751001) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-Tau (T181) antibody (HA751001) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751001) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

Phospho-Tau (T217)
HUABIO
catalog: HA751287

domestic rabbit monoclonal (PSH09-33)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of Phospho-Tau (T217) on different lysates with Rabbit anti-Phospho-Tau (T217) antibody (HA751287) at 1/2,000 dilution. Lane 1: 293T transfected with Tau cell lysate Lane 2: 293T transfected with Tau (mutated T217A) cell lysate (negative) Lysates/proteins at 15 µg/Lane. Predicted band size: 79 kDa Observed band size: 55-75 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751287) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of 293T cells labeling Phospho-Tau (T217) with Rabbit anti-Phospho-Tau (T217) antibody (HA751287) at 1/500 dilution. 293T cells, transfected with empty control (top, negative) / Tau (middle, positive) / Tau (mutated T217A) (bottom, negative) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-Tau (T217) antibody (HA751287) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Western blot analysis of Phospho-Tau (T217) on different lysates with Rabbit anti-Phospho-Tau (T217) antibody (HA751287) at 1/1,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 55-75 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751287) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Tau
HUABIO
catalog: R1401-7

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (R1401-7) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 35-50 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1401-7) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

ICC staining Tau in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tau antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 330 USD
to the supplier