MTAP
HUABIO
catalog: ER1901-47
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of MTAP on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

ICC staining of MTAP in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-47, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MTAP antibody (ER1901-47) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-47) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
MAP2
HUABIO
catalog: HA500177
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of MAP2 on different lysates with Rabbit anti-MAP2 antibody (HA500177) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 200 kDa Observed band size: 300/70 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500177) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of SH-SY5Y cells labeling MAP2 with Rabbit anti-MAP2 antibody (HA500177) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MAP2 antibody (HA500177) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MAP2 antibody (HA500177) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500177) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
MAP2
HUABIO
catalog: HA723025
domestic rabbit monoclonal (PSH08-73)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 500 (MAP2, HA723025, red); 1:1,000 (Iba1, RT1316, green) Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 500 (MAP2, HA723025, red); 1:1,000 (Iba1, RT1316, green) Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1: 500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier
MAP2
HUABIO
catalog: HA751238
domestic rabbit monoclonal (PSH08-73)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 500 (MAP2, HA751238, red); 1:1,000 (Iba1, RT1316, green) Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1: 500 (MAP2, HA751238, red); 1:1,000 (Iba1, RT1316, green) Antigen retrieval: Not required

Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1: 500 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier
MAP2
HUABIO
catalog: HA601459
domestic rabbit polyclonal (PSH08-73)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-MAP2 antibody (HA601459) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601459) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier
MAP2
HUABIO
catalog: HA610293
domestic rabbit polyclonal (PSH08-73)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Application: IHC-Fr Species: Rat Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rat anti-MAP2 antibody (HA610293) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610293) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μg
price: 649.00 USD
to the supplier