LT-alpha
HUABIO
catalog: 0807-5

domestic rabbit polyclonal
reactivity: human, rat
application: western blot

Western blot analysis on LT-alpha (TNF-β) using anti- LT-alpha polyclonal antibody
quantity: 100μl
price: 330 USD
to the supplier

LT-alpha (C-term)
HUABIO
catalog: 0807-6

domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot

Western blot analysis of LT-alpha (C-term) on different lysates with Rabbit anti-LT-alpha (C-term) antibody (0807-6) at 1/2,000 dilution. Lane 1: Ramos cell lysate Lane 2: Mouse skeletal muscle tissue lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 26 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0807-6) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of LT-alpha (C-term) on recombinant Human LTA protein (HA210742) with Rabbit anti-LT-alpha (C-term) antibody (0807-6) at 1/2,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0807-6) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis on LT-alpha(TNF-β) using anti- LT-alpha polyclonal antibody
quantity: 100μl
price: 330 USD
to the supplier

Human TNF-beta
HUABIO
catalog: HA721242

domestic rabbit monoclonal (PS01-05)
reactivity: human
application: ELISA

Standard curve of TNF-β matched pair antibodies: Sandwich ELISA analysis of TNF-β matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721242 [PS01-05] diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant TNF-β protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721243 [PS01-19] (Biotin, 500 ng/ml) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, recombinant TNF-β protein, Streptavidin-HRP are diluted in 1% BSA/PBST.

Western blot analysis of Human TNF-beta on recombinant Human LTA protein (HA210742) with Rabbit anti-Human TNF-beta antibody (HA721242) at 1/2,000 dilution. Lysates/proteins at 10 ng/Lane. Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721242) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Human TNF-beta
HUABIO
catalog: HA721243

domestic rabbit monoclonal (PS01-19)
reactivity: human
application: ELISA

Standard curve of TNF-β matched pair antibodies: Sandwich ELISA analysis of TNF-β matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721242 [PS01-05] diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant TNF-β protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721243 [PS01-19] (Biotin, 500 ng/ml) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, recombinant TNF-β protein, Streptavidin-HRP are diluted in 1% BSA/PBST.

Western blot analysis of Human TNF-beta on recombinant Human LTA protein (HA210742) with Rabbit anti-Human TNF-beta antibody (HA721243) at 1/2,000 dilution. Lysates/proteins at 10 ng/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721243) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

Human TNF-beta
HUABIO
catalog: HA722651B

domestic rabbit monoclonal (PS01-19)
reactivity: human
conjugate: biotin
application: ELISA

quantity: 100μl
price: 409.00 USD
to the supplier

LT-alpha
HUABIO
catalog: M0807-12

mouse monoclonal (C2-F2)
reactivity: human
application: western blot

Western blot analysis on recombinant LT-alpha lysates using anti-LT alpha monoclonal antibody.
quantity: 100μl
price: 360.00 USD
to the supplier

LT-alpha
HUABIO
catalog: M0807-15

mouse monoclonal (6-C10-10)
reactivity: human, rat
application: western blot

Western blot analysis on recombinant LT-alpha (TNF-β) using anti- LT-alpha monoclonal antibody.
quantity: 100μl
price: 360.00 USD
to the supplier