domestic rabbit monoclonal (PSH07-27)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of LAMC2 on different lysates with Rabbit anti-LAMC2 antibody (HA722815) at 1/5,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (low expression) (20 µg/Lane) Lane 3: HepG2 cell lysate (low expression) (20 µg/Lane) Lane 4: HaCaT cell lysate (low expression) (20 µg/Lane) Lane 5: NCI-H441 cell lysate (20 µg/Lane) Lane 6: Mouse skin tissue lysate (40 µg/Lane) Lane 7: Mouse stomach tissue lysate (40 µg/Lane) Lane 8: Rat skin tissue lysate (40 µg/Lane) Lane 9: Rat stomach tissue lysate (40 µg/Lane) Predicted band size: 131 kDa Observed band size: 100/140 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722815) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LAMC2 on different lysates with Rabbit anti-LAMC2 antibody (HA722815) at 1/5,000 dilution. Lane 1: KYSE-150-parental cell lysate Lane 2: KYSE-150-LAMC2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 140/90 kDa Observed band size: 140/90 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722815)) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-LAMC2 antibody (HA722815) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722815) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-14)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human LAMC2 matched pair antibodies Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723717) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human LAMC2 protein (HA211219) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723718, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native LAMC2 in human samples. Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] Interpolated concentration of native LAMC2 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean LAMC2 concentration was determined to be 105,837 pg/mL in A431 and 1,733 pg/ml in A549 cell culture supernatant. There was no detectable signal in HepG2 cell culture supernatant.
Interpolated concentrations of spiked LAMC2 in cell culture media samples. Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] The concentrations of LAMC2 were measured in duplicates, interpolated from the LAMC2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-15)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human LAMC2 matched pair antibodies Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723717) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human LAMC2 protein (HA211219) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723718, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native LAMC2 in human samples. Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] Interpolated concentration of native LAMC2 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean LAMC2 concentration was determined to be 105,837 pg/mL in A431 and 1,733 pg/ml in A549 cell culture supernatant. There was no detectable signal in HepG2 cell culture supernatant.
Interpolated concentrations of spiked LAMC2 in cell culture media samples. Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] The concentrations of LAMC2 were measured in duplicates, interpolated from the LAMC2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-15)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of human LAMC2 matched pair antibodies Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723717) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human LAMC2 protein (HA211219) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA723718, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native LAMC2 in human samples. Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] Interpolated concentration of native LAMC2 was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean LAMC2 concentration was determined to be 105,837 pg/mL in A431 and 1,733 pg/ml in A549 cell culture supernatant. There was no detectable signal in HepG2 cell culture supernatant.
Interpolated concentrations of spiked LAMC2 in cell culture media samples. Capture: HA723717, Human LAMC2 Rabbit mAb [PSH15-14] Detector: HA723718, Human LAMC2 Rabbit mAb [PSH15-15] The concentrations of LAMC2 were measured in duplicates, interpolated from the LAMC2 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-27)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of LAMC2 on different lysates with Rabbit anti-LAMC2 antibody (HA751148) at 1/5,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (low expression) (20 µg/Lane) Lane 3: HepG2 cell lysate (low expression) (20 µg/Lane) Lane 4: HaCaT cell lysate (low expression) (20 µg/Lane) Lane 5: NCI-H441 cell lysate (20 µg/Lane) Lane 6: Mouse skin tissue lysate (40 µg/Lane) Lane 7: Mouse stomach tissue lysate (40 µg/Lane) Lane 8: Rat skin tissue lysate (40 µg/Lane) Lane 9: Rat stomach tissue lysate (40 µg/Lane) Predicted band size: 131 kDa Observed band size: 100/140 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751148) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LAMC2 on different lysates with Rabbit anti-LAMC2 antibody (HA751148) at 1/5,000 dilution. Lane 1: KYSE-150-parental cell lysate Lane 2: KYSE-150-LAMC2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 140/90 kDa Observed band size: 140/90 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751148)) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-LAMC2 antibody (HA751148) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA751148) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier