domestic rabbit monoclonal (JJ0940)
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (ET1701-94) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HUVEC cell lysate (20 µg/Lane) Lane 5: Human kidney tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 100-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (ET1701-94) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si LAMP1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 100-120 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LAMP1 antibody (ET1701-94) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-94) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-39)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722827) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: HeLa cell lysate Lane 7: HeLa cell lysate treated with deglycosylation Lane 8: NIH/3T3 cell lysate Lane 9: NIH/3T3 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 120/44 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722827) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: L6 cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Predicted band size: 44 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Kidney Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (JJ0940)
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA750328) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HUVEC cell lysate (20 µg/Lane) Lane 5: Human kidney tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 100-120 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750328) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA750328) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si LAMP1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 100-120 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750328) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LAMP1 antibody (HA750328) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750328) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-39)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA751160) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: MDA-MB-231 cell lysate Lane 6: HeLa cell lysate Lane 7: HeLa cell lysate treated with deglycosylation Lane 8: NIH/3T3 cell lysate Lane 9: NIH/3T3 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 120/44 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA751160) at 1/2,000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: L6 cell lysate (15 µg/Lane) Lane 3: C6 cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Predicted band size: 44 kDa Observed band size: 100 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751160) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Kidney Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
quantity: 100μl
price: 649.00 USD
to the supplier