HUABIO KRT16 antibody

Cytokeratin 16
HUABIO
catalog: ET1610-17

domestic rabbit monoclonal (SC52-09)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Cytokeratin 16 on different lysates with Rabbit anti-Cytokeratin 16 antibody (ET1610-17) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HaCaT cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A431 cells labeling Cytokeratin 16 with Rabbit anti-Cytokeratin 16 antibody (ET1610-17) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 16 antibody (ET1610-17) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-Cytokeratin 16 antibody (ET1610-17) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-17) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

pan Cytokeratin
HUABIO
catalog: HA600111F

mouse monoclonal (PDH0-10)
reactivity: human

Immunofluorescence analysis of paraffin-embedded human small intestine tissue labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600111F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA600111F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling pan Cytokeratin with Mouse anti-pan Cytokeratin antibody (HA600111F) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA600111F, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Nuclei were counterstained with DAPI (blue).

Cytokeratin 16
HUABIO
catalog: HA720114F

domestic rabbit monoclonal (SC52-09)
reactivity: human

Immunocytochemistry analysis of A431 cells labeling Cytokeratin 16. Cells were fixed in methanol and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with HA720114F at 1/50 dilution Rabbit monoclonal to Cytokeratin 16 (iFluor™ 594)(shown in red). DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720114F Cytokeratin 16 (iFluor™ 594, red) at 1/50 dilution, washed with PBS. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 16. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720114F Cytokeratin 16 (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain.
quantity: 100μl
price: 429.00 USD
to the supplier

Cytokeratin 16
HUABIO
catalog: HA720137F

domestic rabbit monoclonal (SC52-09)
reactivity: human
application: flow cytometry

Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16 (HA720137F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 16 (HA720137F, green) at 1/50 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 16 (HA720137F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 16 (HA720137F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

Immunocytochemistry analysis of HepG2 cells labeling Cytokeratin 16 with Rabbit anti-Cytokeratin 16 antibody (HA720137F) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 16 antibody (HA720137F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
quantity: 100μl
price: 429.00 USD
to the supplier

Cytokeratin 16
HUABIO
catalog: HA720149F

domestic rabbit monoclonal (SC52-09)
reactivity: human

Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 16 (HA720149F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 16 (HA720149F, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 16. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720149F Cytokeratin 16 (iFluor™ 594, red) at 1/50 dilution, washed with PBS. DAPI was used as nuclear counterstain.
quantity: 100μl
price: 429.00 USD
to the supplier

Cytokeratin 16
HUABIO
catalog: HA750203

domestic rabbit monoclonal (SC52-09)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of Cytokeratin 16 on different lysates with Rabbit anti-Cytokeratin 16 antibody (HA750203) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HaCaT cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 16 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750203) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of A431 cells labeling Cytokeratin 16 with Rabbit anti-Cytokeratin 16 antibody (HA750203) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 16 antibody (HA750203) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-Cytokeratin 16 antibody (HA750203) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750203) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier