domestic rabbit monoclonal (SN72-08)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Cytokeratin 1 on different lysates with Rabbit anti-Cytokeratin 1 antibody (ET1611-46) at 1/1,000 dilution. Lane 1: Mouse skin tissue lysate (RIPA lysis) Lane 2: Mouse skin tissue lysate (hot lysis) Lane 3: Rat skin tissue lysate (RIPA lysis) Lane 4: Rat skin tissue lysate (hot lysis) Lysates/proteins at 40 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-46) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Cytokeratin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 1 antibody (ET1611-46) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-46) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (SN72-08)
reactivity: human, mouse, rat
reactivity: human, mouse, rat
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720124F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720124F, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/200 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 429.00 USD
to the supplier
domestic rabbit monoclonal (SN72-08)
reactivity: human, mouse, rat
reactivity: human, mouse, rat
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720127F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720127F, green) at 1/500 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
quantity: 100μl
price: 429.00 USD
to the supplier
domestic rabbit monoclonal (SN72-08)
reactivity: human, mouse, rat
reactivity: human, mouse, rat
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720141F) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720141F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 1 (HA720141F), Cytokeratin 5 (HA720129F) and Cytokeratin 10 (HA720119F). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 1 (HA720141F, red) at 1/400 dilution, Cytokeratin 5 (HA720129F, green) at 1/100 dilution and Cytokeratin 10 (HA720119F, yellow) at 1/400 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
quantity: 100μl
price: 429.00 USD
to the supplier
domestic rabbit monoclonal (SN72-08)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Cytokeratin 1 on different lysates with Rabbit anti-Cytokeratin 1 antibody (HA750256) at 1/1,000 dilution. Lane 1: Mouse skin tissue lysate (RIPA lysis) Lane 2: Mouse skin tissue lysate (hot lysis) Lane 3: Rat skin tissue lysate (RIPA lysis) Lane 4: Rat skin tissue lysate (hot lysis) Lysates/proteins at 40 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750256) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Cytokeratin 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA750256, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293T cell lysate
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Cytokeratin 1 antibody (HA750256) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750256) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier