domestic rabbit monoclonal (JF99-07)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:2,000 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Liver (Negative) Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH18-14)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of NF-H on different lysates with Rabbit anti-NF-H antibody (HA723977) at 1/50,000 dilution. Lane 1: Mouse cerebellum tissue lysate (5 µg/Lane) Lane 2: Mouse spleen tissue lysate (negative) (5 µg/Lane) Lane 3: Mouse brain tissue lysate (40 µg/Lane) Lane 4: Rat cerebellum tissue lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (negative) (20 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 117 kDa Observed band size: 200 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723977) at 1/50,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IF-Tissue Species: Mouse Site: brain (cortex) Sample: Paraffin-embedded section Antibody concentration: 1/500
Application: IF-Tissue Species: Mouse Site: brain (striatum) Sample: Paraffin-embedded section Antibody concentration: 1/500
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (JF99-07)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:2,000 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Liver (Negative) Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Neurofilament on mouse brain tissue lysate using anti-Neurofilament antibody at 1/500 dilution.
Immunocytochemistry analysis of Neuro-2a cells labeling Neurofilament with Rabbit anti-Neurofilament antibody (R1706-17) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Neurofilament antibody (R1706-17) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Neurofilament antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 330 USD
to the supplier