domestic rabbit monoclonal (SU03-42)
reactivity: human, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
reactivity: human, rat
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section
Western blot analysis of VEGF Receptor 2 on different lysates with Rabbit anti-VEGF Receptor 2 antibody (ET1608-33) at 1/1,000 dilution. Lane 1: Human lung tissue lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 152 kDa Observed band size: 152 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-33) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-VEGF Receptor 2 antibody (ET1608-33) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-33) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-VEGF Receptor 2 antibody (ET1608-33) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-33) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-83)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human VEGF Receptor 2 matched pair antibodies Capture: HA725121, Human VEGF Receptor 2 Rabbit mAb [PSH12-83] Detector: HA725122, Human VEGF Receptor 2 Rabbit mAb [PSH12-84] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725121) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGF Receptor 2 protein (HA211025) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725122, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-84)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human VEGF Receptor 2 matched pair antibodies Capture: HA725121, Human VEGF Receptor 2 Rabbit mAb [PSH12-83] Detector: HA725122, Human VEGF Receptor 2 Rabbit mAb [PSH12-84] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725121) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGF Receptor 2 protein (HA211025) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725122, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH12-84)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human VEGF Receptor 2 matched pair antibodies Capture: HA725121, Human VEGF Receptor 2 Rabbit mAb [PSH12-83] Detector: HA725122, Human VEGF Receptor 2 Rabbit mAb [PSH12-84] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725121) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGF Receptor 2 protein (HA211025) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA725122, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier