CD11b
HUABIO
catalog: EM1701-42

mouse monoclonal (4-6)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1701-42) at 1/1,000 dilution. Lane 1: TF-1 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

ICC staining CD11b (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining CD11b (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 360.00 USD
to the supplier

CD11b
HUABIO
catalog: EM1902-35

mouse monoclonal (A2-B5-D2)
reactivity: human, mouse
application: western blot

Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1902-35) at 1/500 dilution. Lane 1: RAW264.7 cell lysate (RIPA lysis) Lane 2: RAW264.7 cell lysate (hot lysis) Lane 3: RAW264.7 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 10 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-35) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD11b on Mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-35, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier

CD11b
HUABIO
catalog: ET1706-04-50UL

domestic rabbit monoclonal (JU93-81)
reactivity: human
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (ET1706-04) at 1/1,000 dilution. Lane 1: TF-1 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (15 µg/Lane) Lane 3: U-937 cell lysate (30 µg/Lane) Lane 4: Jurkat cell lysate (negative) (10 µg/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fluorescence multiplex immunohistochemical analysis of the human gastric cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD31 (M1511-8, red), anti-αSMA (ET1607-53, gray), anti-CD11b (ET1706-04, cyan), anti-panCK (HA601138, magenta) and anti-CD3 (HA720082, yellow) on human gastric cancer. Panel B: anti- CD31 stained on the endothelial cells. Panel C: anti-αSMA stained on cancer-associated fibroblasts and smooth muscle cells. Panel D: anti-CD11b stained on myeloid cells. Panel E: anti-panCK stained on cancer cells. Panel F: anti-CD3 stained on T cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in five rounds of staining: in the order of M1511-8 (1/1,000 dilution), ET1607-53 (1/2,000 dilution), ET1706-04 (1/1,000 dilution), HA601138 (1/3,000 dilution), and HA720082 (1/500 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Fluorescence multiplex immunohistochemical analysis of human gastric cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD11b (ET1706-04, Red), anti-CD3 (HA720082, Green) and anti-CD31 (M1511-8, Yellow) on human gastric cancer. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1706-04 (1/1,000 dilution), HA720082 (1/500 dilution) and M1511-8 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.
quantity: 50μl
price: 205.00 USD
to the supplier

Human CD11b
HUABIO
catalog: HA600130F

mouse monoclonal (A2-9-R)
reactivity: human
conjugate: FITC
application: flow cytometry

Flow cytometric analysis of human peripheral blood labelling Human CD11b (HA600130F, FITC).
quantity: 100μl
price: 394.00 USD
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CD11b
HUABIO
catalog: HA722075

domestic rabbit monoclonal (PSH03-96)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA722075) at 1/2,000 dilution. Lane 1: TF-1 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (15 µg/Lane) Lane 3: U-937 cell lysate (30 µg/Lane) Lane 4: Jurkat cell lysate (negative) (10 µg/Lane) Lane 5: RAW264.7 cell lysate (5 µg/Lane) Lane 6: J774A.1 cell lysate (5 µg/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722075) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA722075) at 1/2,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (negative) Lane 3: Rat spleen tissue lysate Lane 4: Rat heart tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722075) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-CD11b antibody (HA722075) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722075) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

CD11b
HUABIO
catalog: HA722856

domestic rabbit monoclonal (JE01-17)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA722856) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate (5 µg/Lane) Lane 2: C2C12 cell lysate (negative) (10 µg/Lane) Lane 3: J774A.1 cell lysate (5 µg/Lane) Lane 4: Mouse spleen tissue lysate (20 µg/Lane) Lane 5: Mouse heart tissue lysate (negative) (20 µg/Lane) Lane 6: Rat spleen tissue lysate (20 µg/Lane) Lane 7: Rat heart tissue lysate (negative) (20 µg/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722856) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-CD11b antibody (HA722856) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722856, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunofluorescence analysis of frozen rat spleen tissue with Rabbit anti-CD11b antibody (HA722856) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722856, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier

CD11b
HUABIO
catalog: HA750451

domestic rabbit monoclonal (JU93-81)
reactivity: human
application: western blot, immunoprecipitation, immunohistochemistry - paraffin section

Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA750451) at 1/1,000 dilution. Lane 1: TF-1 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (15 µg/Lane) Lane 3: U-937 cell lysate (30 µg/Lane) Lane 4: Jurkat cell lysate (negative) (10 µg/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750451) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labelling CD11 b (HA750451). The human lymph node section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes, blocked in 10% goat serum, and then incubated with ET1706-04 at 1/50 dilution , followed by iFluorTM 488 Conjugated Goat anti-rabbit IgG at 1:1000 dilution. Nuclear was stained with Hoechst 33258 at 1/5,000 dilution. Confocal images shows specific membrane staining of CD11b in human lymph node.

Immunofluorescence analysis of paraffin-embedded human spleen tissue labelling CD11 b (HA750451). The human spleen section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes, blocked in 10% goat serum, and then incubated with ET1706-04 at 1/50 dilution , followed by iFluorTM 488 Conjugated Goat anti-rabbit IgG at 1:1000 dilution. Nuclear was stained with Hoechst 33258 at 1/5,000 dilution. Confocal images shows specific membrane staining of CD11b in human spleen.
quantity: 100μl
price: 649.00 USD
to the supplier

CD11b
HUABIO
catalog: HA750921

domestic rabbit monoclonal (PSH03-96)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA750921) at 1/2,000 dilution. Lane 1: TF-1 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (15 µg/Lane) Lane 3: U-937 cell lysate (30 µg/Lane) Lane 4: Jurkat cell lysate (negative) (10 µg/Lane) Lane 5: RAW264.7 cell lysate (5 µg/Lane) Lane 6: J774A.1 cell lysate (5 µg/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750921) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA750921) at 1/2,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse heart tissue lysate (negative) Lane 3: Rat spleen tissue lysate Lane 4: Rat heart tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750921) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-CD11b antibody (HA750921) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750921) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier

CD11b
HUABIO
catalog: IRS014

domestic rabbit monoclonal
reactivity: human

mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11b (IRS014), CD31 (IRS023), aSMA (IRS012), panCK (IRS010) and CD3 (IRS022) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11b antibody (IRS014) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
quantity: 100μl
price: 385.00 USD
to the supplier

CD11b
HUABIO
catalog: M1211-6

mouse monoclonal (A2-9)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lysates/proteins at 10 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-6) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD11b antibody (M1211-6) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1211-6) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 360.00 USD
to the supplier