IL-17
HUABIO
catalog: ER1706-91
domestic rabbit polyclonal
reactivity: human, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of IL-17 on IL-17 recombinant protein with Rabbit anti-IL-17 antibody (ER1706-91) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Predicted band size: 18 kDa Observed band size: 16 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-91) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IL-17 antibody (ER1706-91) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-91) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow cytometric analysis of Jurkat cells with IL-17 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier
IL-17A
HUABIO
catalog: ER1902-37
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of IL-17A on recombinant protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-37, 1/20,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IL-17A on Rat stomach tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IL-17A on Human skeletal muscle tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
Human IL-17A
HUABIO
catalog: HA721232
domestic rabbit monoclonal (PS01-20)
reactivity: human
application: ELISA

Sandwich ELISA analysis of IL-17A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721232 [PS01-20] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-17A protein (HA210731) starting from 2,000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721233 [PS01-24] (Biotin, 1/2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, Recombinant IL-17A protein, Streptavidin-HRP are diluted in 1%BSA/PBST.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-17A
HUABIO
catalog: HA721233
domestic rabbit monoclonal (PS01-24)
reactivity: human
application: ELISA

Sandwich ELISA analysis of IL-17A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721232 [PS01-20] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-17A protein (HA210731) starting from 2,000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721233 [PS01-24] (Biotin, 1/2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, Recombinant IL-17A protein, Streptavidin-HRP are diluted in 1%BSA/PBST.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-17A
HUABIO
catalog: HA721296
domestic rabbit monoclonal (PS01-24)
reactivity: human
conjugate: HRP
application: ELISA

Advantages of Using rAbs： Increased Reproducibility Because recombinant antibody production involves sequencing the antibody light and heavy chains, recombinant antibody production allows researchers more control over the antigen. Ease of Scalability and Continuous Supply In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. Recombinant antibodies can be produced in weeks as opposed to months. Animal-Free Tech Once the antibody-producing genes are isolated, high-throughput in vitro manufacture can be implemented. This eliminates the numerous ethical and animal welfare concerns commonly associated with traditional monoclonal antibody production.
quantity: 100μl
price: 409.00 USD
to the supplier
Human IL-17A
HUABIO
catalog: HA721631
domestic rabbit monoclonal (PS01-24)
reactivity: human
conjugate: biotin
application: ELISA

Advantages of Using rAbs： Increased Reproducibility Because recombinant antibody production involves sequencing the antibody light and heavy chains, recombinant antibody production allows researchers more control over the antigen. Ease of Scalability and Continuous Supply In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. Recombinant antibodies can be produced in weeks as opposed to months. Animal-Free Tech Once the antibody-producing genes are isolated, high-throughput in vitro manufacture can be implemented. This eliminates the numerous ethical and animal welfare concerns commonly associated with traditional monoclonal antibody production.
quantity: 100μl
price: 409.00 USD
to the supplier