domestic rabbit monoclonal (PSH03-85)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of IL-13R alpha 2 on different lysates with Rabbit anti-IL-13R alpha 2 antibody (HA722051) at 1/2,000 dilution. Lane 1: A375 cell lysate (no heat) Lane 2: U-937 cell lysate (no heat) (negative) Lane 3: Daudi cell lysate (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 50-80 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722051) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IL-13R alpha 2 on U-87 MG cell lysates with Rabbit anti-IL-13R alpha 2 antibody (HA722051) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 50-70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722051) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-IL-13R alpha 2 antibody (HA722051) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722051) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-63)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human IL-13 R alpha 2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722880) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rrecombinant Human IL-13R alpha 2 protein (HA210969) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA722881, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native human IL-13 R alpha 2 in U-87 MG extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG extract 50%. The mean human IL-13 R alpha 2 concentration was determined to be 4,569 pg/ml in U-87 MG extract.
Interpolated concentrations of native human IL-13 R alpha 2 in A375 extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are A375 extract 10%. The mean human IL-13 R alpha 2 concentration was determined to be 123.1 ng/ml in A375 extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-64)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human IL-13 R alpha 2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722880) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rrecombinant Human IL-13R alpha 2 protein (HA210969) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA722881, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native human IL-13 R alpha 2 in U-87 MG extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG extract 50%. The mean human IL-13 R alpha 2 concentration was determined to be 4,569 pg/ml in U-87 MG extract.
Interpolated concentrations of native human IL-13 R alpha 2 in A375 extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are A375 extract 10%. The mean human IL-13 R alpha 2 concentration was determined to be 123.1 ng/ml in A375 extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-64)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human IL-13 R alpha 2 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722880) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Rrecombinant Human IL-13R alpha 2 protein (HA210969) starting from 18,000 pg/ml to 0 pg/ml and detect antibody (HA722882B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native human IL-13 R alpha 2 in U-87 MG extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are U-87 MG extract 50%. The mean human IL-13 R alpha 2 concentration was determined to be 4,569 pg/ml in U-87 MG extract.
Interpolated concentrations of native human IL-13 R alpha 2 in A375 extract samples based on a 1,000 µg/ml extract load. The concentrations of human IL-13 R alpha 2 were interpolated from the human IL-13 R alpha 2 standard curve and corrected for sample dilution. Undiluted samples are A375 extract 10%. The mean human IL-13 R alpha 2 concentration was determined to be 123.1 ng/ml in A375 extract.
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH03-85)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of IL-13R alpha 2 on different lysates with Rabbit anti-IL-13R alpha 2 antibody (HA750910) at 1/2,000 dilution. Lane 1: A375 cell lysate (no heat) Lane 2: U-937 cell lysate (no heat) (negative) Lane 3: Daudi cell lysate (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 50-80 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750910) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IL-13R alpha 2 on U-87 MG cell lysates with Rabbit anti-IL-13R alpha 2 antibody (HA750910) at 1/2,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 44 kDa Observed band size: 50-70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750910) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-IL-13R alpha 2 antibody (HA750910) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750910) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 649.00 USD
to the supplier