domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry
reactivity: human
application: western blot, flow cytometry
Western blot analysis of IL-10RB on different lysates with Rabbit anti-IL-10RB antibody (HA500322) at 1/500 dilution. Lane 1: 293T cell lysate Lane 2: Jurkat cell lysate Lane 3: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 37 kDa Observed band size: 42 kDa (Glycoprotein) Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500322) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of SiHa cells labeling IL-10RB with Rabbit anti-IL-10RB antibody (HA500322) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-10RB antibody (HA500322) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Flow cytometric analysis of THP-1 cells labeling IL-10RB. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500322, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier
mouse monoclonal (A8E11)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of IL-10RB on different lysates with Mouse anti-IL-10RB antibody (HA601061) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: 293T cell lysate Lane 3: U-87 MG cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 37 kDa Observed band size: 42 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601061) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-44)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human IL-10RB matched pair antibodies Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44] Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA725295 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-10RB (HA211205) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725296, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IL-10RB in THP-1 cell extract: Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44] Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45] The concentrations of IL-10RB were measured in duplicates, interpolated from the IL-10RB standard curve and corrected for sample dilution. Undiluted samples are THP-1 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10RB concentration was determined to be 861.6 pg/ml in THP-1 cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-45)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human IL-10RB matched pair antibodies Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44] Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA725295 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-10RB (HA211205) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725296, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IL-10RB in THP-1 cell extract: Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44] Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45] The concentrations of IL-10RB were measured in duplicates, interpolated from the IL-10RB standard curve and corrected for sample dilution. Undiluted samples are THP-1 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10RB concentration was determined to be 861.6 pg/ml in THP-1 cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH17-45)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human IL-10RB matched pair antibodies Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44] Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA725295 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-10RB (HA211205) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725296, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IL-10RB in THP-1 cell extract: Capture: HA725295, Human IL-10RB Rabbit mAb [PSH17-44] Detector: HA725296, Human IL-10RB Rabbit mAb [PSH17-45] The concentrations of IL-10RB were measured in duplicates, interpolated from the IL-10RB standard curve and corrected for sample dilution. Undiluted samples are THP-1 cell extract 250 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-10RB concentration was determined to be 861.6 pg/ml in THP-1 cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier