IL-8
HUABIO
catalog: ER1706-67

domestic rabbit polyclonal
reactivity: human
application: ELISA, immunohistochemistry - paraffin section

ICC staining IL-8 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining IL-8 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining IL-8 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier

IL-8
HUABIO
catalog: ER1901-61

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (ER1901-61) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-61) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of U-87 MG cells treated with 1μM Thapsigargin for 24 hours labeling IL-8 with Rabbit anti-IL-8 antibody (ER1901-61) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-8 antibody (ER1901-61) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-61, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier

Human IL-8
HUABIO
catalog: HA722098

domestic rabbit monoclonal (PS00-83)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human IL-8 matched pair antibodies Capture: HA722098, Human IL-8 Rabbit mAb [PS00-83] Detector: HA722099, Human IL-8 Rabbit mAb [PS01-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722098) diluted in carbonate/bicarbonate buffer, at a concentration of 0.01 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-8 protein (HA210642) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722099, Biotin, 0.01 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Human IL-8
HUABIO
catalog: HA722099

domestic rabbit monoclonal (PS01-22)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human IL-8 matched pair antibodies Capture: HA722098, Human IL-8 Rabbit mAb [PS00-83] Detector: HA722099, Human IL-8 Rabbit mAb [PS01-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722098) diluted in carbonate/bicarbonate buffer, at a concentration of 0.01 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-8 protein (HA210642) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722099, Biotin, 0.01 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Human IL-8
HUABIO
catalog: HA722611B

domestic rabbit monoclonal (PS01-22)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human IL-8 matched pair antibodies Capture: HA722098, Human IL-8 Rabbit mAb [PS00-83] Detector: HA722099, Human IL-8 Rabbit mAb [PS01-22] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722098) diluted in carbonate/bicarbonate buffer, at a concentration of 0.01 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-8 protein (HA210642) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722099, Biotin, 0.01 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

IL-8
HUABIO
catalog: HA723263

domestic rabbit monoclonal (PSH10-96)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (HA723263) at 1/2,000 dilution. Lane 1: U-937 cell lysate Lane 2: U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723263) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of U-937 cells untreated (left) / treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours (right) labeling IL-8. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723263, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

IL-8
HUABIO
catalog: HA723264

domestic rabbit monoclonal (PSH10-97)
reactivity: human
application: western blot

Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (HA723264) at 1/2,000 dilution. Lane 1: U-937 cell lysate Lane 2: U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate Lane 3: U-87 MG cell lysate Lane 4: U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723264) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

IL-8
HUABIO
catalog: HA751386

domestic rabbit monoclonal (PSH10-96)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (HA751386) at 1/2,000 dilution. Lane 1: U-937 cell lysate Lane 2: U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751386) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of U-937 cells untreated (left) / treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours (right) labeling IL-8. Cells were fixed and permeabilized. Then stained with the primary antibody (HA751386, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier

IL-8
HUABIO
catalog: HA751387

domestic rabbit monoclonal (PSH10-97)
reactivity: human
application: western blot

Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (HA751387) at 1/2,000 dilution. Lane 1: U-937 cell lysate Lane 2: U-937 treated with 100ng/mL TPA for 24 hours then add 5μg/mL LPS for 4 hours then add 300ng/mL BFA for 3 hours cell lysate Lane 3: U-87 MG cell lysate Lane 4: U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751387) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier

IL-8
HUABIO
catalog: R1511-15

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of IL-8 on different lysates with Rabbit anti-IL-8 antibody (R1511-15) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG treated with 1μM Thapsigargin for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-15) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

ICC staining IL-8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow cytometric analysis of HUVEC cells with IL-8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
quantity: 100μl
price: 330 USD
to the supplier