domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
domestic rabbit polyclonal
reactivity: human, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, zebrafish
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of IL-7R-alpha on zebrafish tissue lysate using anti-IL-7R-alpha antibody at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-IL-7R-alpha antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Daudi cells with IL-7R-alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier
mouse monoclonal (PSH12-76)
reactivity: human
application: flow cytometry
reactivity: human
application: flow cytometry
Flow cytometric analysis of human peripheral blood lymphocytes labeling CD127. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601429, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 360.00 USD
to the supplier
mouse monoclonal (PSH12-76)
reactivity: human
conjugate: APC
application: flow cytometry
reactivity: human
conjugate: APC
application: flow cytometry
Flow cytometric analysis of human peripheral blood cells labeling CD127 (HA601536F, APC). Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with CD127 (HA601536F, APC, 1/1,000) compared with Mouse IgG Isotype Control (APC, 1/1,000).
quantity: 100T
price: 539.00 USD
to the supplier
domestic rabbit monoclonal (JE37-10)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of CD127 on K562 cell lysates with Rabbit anti-CD127 antibody (HA721214) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721214) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD127 on different lysates with Rabbit anti-CD127 antibody (HA721214) at 1/1,000 dilution. Lane 1: ID8-si NT cell lysate Lane 2: ID8-si CD127 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721214) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of SiHa cells labeling CD127 with Rabbit anti-CD127 antibody (HA721214) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD127 antibody (HA721214) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 385.00 USD
to the supplier