domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section
reactivity: human
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of IL-6R on different lysates with Rabbit anti-IL-6R antibody (HA500438) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: A549 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 52 kDa Observed band size: 47 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500438) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ICC staining of IL-6R in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500438, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-IL-6R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500438, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH15-38)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human IL-6R matched pair antibodies Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723743) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-6R protein (HA210640) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723744, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IL-6R in THP-1 and Daudi cell culture supernatant . Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] Interpolated concentration of native IL-6R was measured in duplicate at different sample concentrations and interpolated from the IL-6R standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-6R concentration was determined to be 3824pg/mL in THP-1 cell culture supernatan, undetectable in Daudi cell culture supernatan.
Interpolated concentrations of spiked IL-6R in cell culture media samples. Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] The concentrations of IL-6R were measured in duplicates, interpolated from the IL-6R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-39)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human IL-6R matched pair antibodies Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723743) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-6R protein (HA210640) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723744, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IL-6R in THP-1 and Daudi cell culture supernatant . Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] Interpolated concentration of native IL-6R was measured in duplicate at different sample concentrations and interpolated from the IL-6R standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-6R concentration was determined to be 3824pg/mL in THP-1 cell culture supernatan, undetectable in Daudi cell culture supernatan.
Interpolated concentrations of spiked IL-6R in cell culture media samples. Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] The concentrations of IL-6R were measured in duplicates, interpolated from the IL-6R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-39)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Human IL-6R matched pair antibodies Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723743) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-6R protein (HA210640) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723744, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IL-6R in THP-1 and Daudi cell culture supernatant . Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] Interpolated concentration of native IL-6R was measured in duplicate at different sample concentrations and interpolated from the IL-6R standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean IL-6R concentration was determined to be 3824pg/mL in THP-1 cell culture supernatan, undetectable in Daudi cell culture supernatan.
Interpolated concentrations of spiked IL-6R in cell culture media samples. Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] The concentrations of IL-6R were measured in duplicates, interpolated from the IL-6R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
domestic rabbit monoclonal (PSH15-39)
reactivity: human
conjugate: HRP
application: ELISA
reactivity: human
conjugate: HRP
application: ELISA
Sandwich ELISA analysis of Human IL-6R matched pair antibodies Capture: HA723743, Human IL-6R Rabbit mAb [PSH15-38] Detector: HA723744, Human IL-6R Rabbit mAb [PSH15-39] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723743) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-6R protein (HA210640) starting from 33,000 pg/ml to 0 pg/ml and detect antibody (HA723744, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier