Human IL-6
HUABIO
catalog: HA601050
mouse monoclonal (A6D2)
reactivity: human
application: western blot, ELISA

Western blot analysis of Human IL-6 on recombinant protein with Mouse anti-Human IL-6 antibody (HA601050) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601050) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Human IL-6 Antibody (HA601050) in indirect ELISA. Indirect ELISA analysis of Human IL-6 was performed by coating wells of a 96-well plate with 50 µl per well of Human IL-6 antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse Human IL-6 monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 360.00 USD
to the supplier
Human IL-6
HUABIO
catalog: HA601051
mouse monoclonal (A6D1)
reactivity: human
application: western blot, ELISA

Western blot analysis of Human IL-6 on different lysates with Mouse anti-Human IL-6 antibody (HA601051) at 1/500 dilution. Lane 1: SiHa cell lysate Lane 2: A549 cell lysate Lane 3: Hela cell lysate Lane 4: Human lung tissue lysate (20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601051) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Human IL-6 on recombinant protein with Mouse anti-Human IL-6 antibody (HA601051) at 1/500 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601051) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
Human IL-6
HUABIO
catalog: HA721167
domestic rabbit monoclonal (PS00-21)
reactivity: human
application: ELISA
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-6
HUABIO
catalog: HA721168
domestic rabbit monoclonal (PS00-15)
reactivity: human
application: ELISA
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-6
HUABIO
catalog: HA721293
domestic rabbit monoclonal (PS00-15)
reactivity: human
conjugate: HRP
application: ELISA

Dilute the Capture Antibody (HA721167) to the working concentration 5μg/ml in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at 4℃. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Add human IL6 protein (HA210797) with 1:1 serially diluted starting from a concentration of 5,000pg/ml and incubate 45 minutes at room temperature. Add 100 μL of the HRP-conjugated Detection Antibody (PS00-15) at a dilution of 0.2μg/ml to each well. Cover the plate and incubate for 30 minutes at room temperature. Add 50 μL of Stop Solution to each well. Determine the optical density of each well immediately, using a microplate reader set to 450 nm.

Advantages of Using rAbs： Increased Reproducibility Because recombinant antibody production involves sequencing the antibody light and heavy chains, recombinant antibody production allows researchers more control over the antigen. Ease of Scalability and Continuous Supply In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. Recombinant antibodies can be produced in weeks as opposed to months. Animal-Free Tech Once the antibody-producing genes are isolated, high-throughput in vitro manufacture can be implemented. This eliminates the numerous ethical and animal welfare concerns commonly associated with traditional monoclonal antibody production.
quantity: 100μl
price: 409.00 USD
to the supplier
Human IL-6
HUABIO
catalog: HA722233B
domestic rabbit monoclonal (PS00-15)
reactivity: human
conjugate: biotin
application: ELISA
quantity: 100μl
price: 409.00 USD
to the supplier
IL-6
HUABIO
catalog: R1412-2
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section

Western blot analysis of IL-6 on recombinant murine Interleukin-6 protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1412-2, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

ICC staining of IL-6 in Siha cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1412-2, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1412-2, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier