domestic rabbit polyclonal
reactivity: human
application: western blot, ELISA
reactivity: human
application: western blot, ELISA
Western blot analysis of IL-5 on LOVO cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500081, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ICC staining of IL-5 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500081, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH04-64)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human IL-5 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722164) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-5 protein (HA210594) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA722165, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Conditioned media was harvested after 6 days. IL-5 was measured in 100% unstimulated and PHA-M stimulated human PBMC cell supernatant. The concentrations of IL-5 were interpolated from the IL-5 standard curves. The mean IL-5 concentration was determined to be 475pg/mL in PHA-M stimulated human PBMC cell supernatant. There was no detectable signal in unstimulated supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH04-65)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of Human IL-5 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722164) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-5 protein (HA210594) starting from 1000 pg/ml to 0 pg/ml and detect antibody (HA722165, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Conditioned media was harvested after 6 days. IL-5 was measured in 100% unstimulated and PHA-M stimulated human PBMC cell supernatant. The concentrations of IL-5 were interpolated from the IL-5 standard curves. The mean IL-5 concentration was determined to be 475pg/mL in PHA-M stimulated human PBMC cell supernatant. There was no detectable signal in unstimulated supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH04-65)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA