IL-4R
HUABIO
catalog: HA500453
domestic rabbit polyclonal
reactivity: human
application: western blot

Western blot analysis of IL-4R on PC-3M cell lysates with Rabbit anti-IL-4R antibody (HA500453) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 90 kDa Observed band size: 140 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500453) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of PC-3M cells labeling IL-4R with Rabbit anti-IL-4R antibody (HA500453) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-4R antibody (HA500453) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
quantity: 100μl
price: 330 USD
to the supplier
IL-4R
HUABIO
catalog: HA500454
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of IL-4R on different lysates with Rabbit anti-IL-4R antibody (HA500454) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Daudi cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: Ramos cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: Mouse spleen tissue lysate (40 µg/Lane) Lane 10: Mouse pancreas tissue lysate (40 µg/Lane) Lane 11: Rat pancreas tissue lysate (40 µg/Lane) Predicted band size: 90 kDa Observed band size: 110 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500454) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-IL-4R antibody (HA500454) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500454) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Application: IF-Tissue Species: Human Site: tonsil Sample: Paraffin-embedded section Antibody concentration: 1/500
quantity: 100μl
price: 330 USD
to the supplier
IL-4R
HUABIO
catalog: HA723253
domestic rabbit monoclonal (PSH10-86)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of IL-4R on different lysates with Rabbit anti-IL-4R antibody (HA723253) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Daudi cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: Ramos cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: PC-12 cell lysate (20 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Lane 11: Mouse pancreas tissue lysate (40 µg/Lane) Predicted band size: 90 kDa Observed band size: 110 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723253) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

IL-4R was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA723253 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723253 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA723253 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA723253 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 51 seconds; ECL: K1801

Immunocytochemistry analysis of PC-12 cells labeling IL-4R with Rabbit anti-IL-4R antibody (HA723253) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-4R antibody (HA723253) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
Human IL-4R
HUABIO
catalog: HA723336
domestic rabbit monoclonal (PSH11-45)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human IL-4R matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723336) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-4R protein (HA210720) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723337, Biotin, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-4R in Daudi and MCF7 extract samples based on a 1,000 µg/ml extract load. The concentrations of IL-4R were measured in duplicates, interpolated from the IL-4R standard curve and corrected for sample dilution. Undiluted samples are Daudi extract 100% and MCF7 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4R concentration was determined to be 1,789 pg/ml in Daudi extract and undetectable in MCF7 extract.

Interpolated concentrations of spiked IL-4R in human cell culture media samples. The concentrations of IL-4R were measured in duplicates, interpolated from the IL-4R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-4R
HUABIO
catalog: HA723337
domestic rabbit monoclonal (PSH11-46)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human IL-4R matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723336) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-4R protein (HA210720) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723337, Biotin, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-4R in Daudi and MCF7 extract samples based on a 1,000 µg/ml extract load. The concentrations of IL-4R were measured in duplicates, interpolated from the IL-4R standard curve and corrected for sample dilution. Undiluted samples are Daudi extract 100% and MCF7 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4R concentration was determined to be 1,789 pg/ml in Daudi extract and undetectable in MCF7 extract.

Interpolated concentrations of spiked IL-4R in human cell culture media samples. The concentrations of IL-4R were measured in duplicates, interpolated from the IL-4R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-4R
HUABIO
catalog: HA723338B
domestic rabbit monoclonal (PSH11-46)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human IL-4R matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723336) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-4R protein (HA210720) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA723338B, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IL-4R in Daudi and MCF7 extract samples based on a 1,000 µg/ml extract load. The concentrations of IL-4R were measured in duplicates, interpolated from the IL-4R standard curve and corrected for sample dilution. Undiluted samples are Daudi extract 100% and MCF7 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4R concentration was determined to be 1,789 pg/ml in Daudi extract and undetectable in MCF7 extract.

Interpolated concentrations of spiked IL-4R in human cell culture media samples. The concentrations of IL-4R were measured in duplicates, interpolated from the IL-4R standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
IL-4R
HUABIO
catalog: HA751378
domestic rabbit monoclonal (PSH10-86)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation

Western blot analysis of IL-4R on different lysates with Rabbit anti-IL-4R antibody (HA751378) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Daudi cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: K-562 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: MCF7 cell lysate (20 µg/Lane) Lane 7: Ramos cell lysate (20 µg/Lane) Lane 8: RAW264.7 cell lysate (20 µg/Lane) Lane 9: PC-12 cell lysate (20 µg/Lane) Lane 10: Mouse spleen tissue lysate (40 µg/Lane) Lane 11: Mouse pancreas tissue lysate (40 µg/Lane) Predicted band size: 90 kDa Observed band size: 110 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

IL-4R was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA751378 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751378 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA751378 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA751378 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 51 seconds; ECL: K1801

Immunocytochemistry analysis of PC-12 cells labeling IL-4R with Rabbit anti-IL-4R antibody (HA751378) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-4R antibody (HA751378) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 649.00 USD
to the supplier