IL-4
HUABIO
catalog: ER1706-55
domestic rabbit polyclonal
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of IL4 on recombiant protein lysate using anti-IL4 antibody at 1/1,000 dilution.

Immunocytochemistry analysis of Ramos cells labeling IL-4 with Rabbit anti-IL-4 antibody (ER1706-55) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-4 antibody (ER1706-55) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-IL4 antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 330 USD
to the supplier
IL-4
HUABIO
catalog: ET1704-28
domestic rabbit monoclonal (JA86-10)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

ICC staining of IL-4 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of IL-4 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ICC staining of IL-4 in D3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1704-28, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier
IL-4
HUABIO
catalog: HA601034
mouse monoclonal (A7D7)
reactivity: human
application: ELISA

IL-4 Antibody (HA601034) in indirect ELISA. Indirect ELISA analysis of IL-4 was performed by coating wells of a 96-well plate with 50 µl per well of IL-4 antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse IL-4 monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 360.00 USD
to the supplier
Human IL-4
HUABIO
catalog: HA721230
domestic rabbit monoclonal (PS00-67)
reactivity: human
application: ELISA

Sandwich ELISA analysis of IL-4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721230 [PS00-67] diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1%BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-4 protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721231 [PS00-66] (Biotin, 1:4000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, Recombinant IL-4 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-4
HUABIO
catalog: HA721231
domestic rabbit monoclonal (PS00-66)
reactivity: human
application: ELISA

Sandwich ELISA analysis of IL-4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721230 [PS00-67] diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1%BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-4 protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721368 [PS00-66] (Biotin, 1:4000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, Recombinant IL-4 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.
quantity: 100μl
price: 649.00 USD
to the supplier
Human IL-4
HUABIO
catalog: HA721295
domestic rabbit monoclonal (PS00-66)
reactivity: human
conjugate: HRP
application: ELISA

Advantages of Using rAbs： Increased Reproducibility Because recombinant antibody production involves sequencing the antibody light and heavy chains, recombinant antibody production allows researchers more control over the antigen. Ease of Scalability and Continuous Supply In vitro methods for producing antibodies are amenable to large-scale production, meaning antibody availability is unlikely to become a limiting factor. Recombinant antibodies can be produced in weeks as opposed to months. Animal-Free Tech Once the antibody-producing genes are isolated, high-throughput in vitro manufacture can be implemented. This eliminates the numerous ethical and animal welfare concerns commonly associated with traditional monoclonal antibody production.
quantity: 100μl
price: 409.00 USD
to the supplier
Human IL-4
HUABIO
catalog: HA721368
domestic rabbit monoclonal (PS00-66)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of IL-4 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721230 [PS00-67] diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1%BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-4 protein starting from 2000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721368 [PS00-66] (Biotin, 1:4000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, Recombinant IL-4 protein, Streptavidin-HRP are diluted in 1% BSA/PBST.
quantity: 100μl
price: 409.00 USD
to the supplier