Fas / CD95
HUABIO
catalog: ER1902-56

domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot analysis of Fas / CD95 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-56, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of Fas / CD95 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-56, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier

Fas / CD95
HUABIO
catalog: ET1701-92

domestic rabbit monoclonal (JJ0942)
reactivity: human
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of Fas / CD95 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: Hela cell lysate

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Fas / CD95 antibody (ET1701-92) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-92) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Fas / CD95 antibody (ET1701-92) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-92) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier

Human Fas / CD95
HUABIO
catalog: HA723835

domestic rabbit monoclonal (PSH16-37)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Fas / CD95 matched pair antibodies Capture: HA723835, Human Fas/CD95 Rabbit mAb [PSH16-37] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723835) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fas / CD95 protein (HA211064) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723836, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native FAS in Raji，Ramos and K-562 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723835, Human Fas/CD95 Rabbit mAb [PSH16-37] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Interpolated concentration of native FAS was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 25,215 pg/mL in Raji Cell extract , 1,597 pg/mL in Ramos Cell extract and undetectable in K-562 Cell extract.

Interpolated concentrations of spiked FAS in cell culture media samples. Capture: HA723835, Human Fas/CD95 Rabbit mAb [PSH16-37] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] The concentrations of FAS were measured in duplicates, interpolated from the FAS standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier

Human Fas / CD95
HUABIO
catalog: HA723836

domestic rabbit monoclonal (PSH16-38)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Fas / CD95 matched pair antibodies Capture: HA723835, Human Fas/CD95 Rabbit mAb [PSH16-37] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723835) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fas / CD95 protein (HA211064) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723836, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Fas / CD95 matched pair antibodies Capture: HA723837, Human Fas/CD95 Rabbit mAb [PSH16-39] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723837) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fas / CD95 protein (HA211064) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723836, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native FAS in Raji，Ramos and K-562 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723835, Human Fas/CD95 Rabbit mAb [PSH16-37] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Interpolated concentration of native FAS was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 25,215 pg/mL in Raji Cell extract , 1,597 pg/mL in Ramos Cell extract and undetectable in K-562 Cell extract.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Fas / CD95
HUABIO
catalog: HA723836B

domestic rabbit monoclonal (PSH16-38)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Fas / CD95 matched pair antibodies Capture: HA723835, Human Fas/CD95 Rabbit mAb [PSH16-37] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723835) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fas / CD95 protein (HA211064) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723836, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Sandwich ELISA analysis of Human Fas / CD95 matched pair antibodies Capture: HA723837, Human Fas/CD95 Rabbit mAb [PSH16-39] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723837) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fas / CD95 protein (HA211064) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723836, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native FAS in Raji，Ramos and K-562 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723835, Human Fas/CD95 Rabbit mAb [PSH16-37] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Interpolated concentration of native FAS was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 25,215 pg/mL in Raji Cell extract , 1,597 pg/mL in Ramos Cell extract and undetectable in K-562 Cell extract.
quantity: 100μl
price: 409.00 USD
to the supplier

Human Fas / CD95
HUABIO
catalog: HA723837

domestic rabbit monoclonal (PSH16-39)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Fas / CD95 matched pair antibodies Capture: HA723837, Human Fas/CD95 Rabbit mAb [PSH16-39] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723837) diluted in carbonate/bicarbonate buffer, at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Fas / CD95 protein (HA211064) starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA723836, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native FAS in Raji，Ramos and K-562 Cell extract samples based on a 1000 µg/ml extract load. Capture: HA723837, Human Fas/CD95 Rabbit mAb [PSH16-39] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] Interpolated concentration of native FAS was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FAS concentration was determined to be 15,671 pg/mL in Raji Cell extract 1,643 pg/mL in Ramos Cell extract and undetectable in K-562 Cell extract.

Interpolated concentrations of spiked FAS in cell culture media samples. Capture: HA723837, Human Fas/CD95 Rabbit mAb [PSH16-39] Detector: HA723836, Human Fas/CD95 Rabbit mAb [PSH16-38] The concentrations of FAS were measured in duplicates, interpolated from the FAS standard curves and corrected for sample dilution. diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier