IFNGR1
HUABIO
catalog: ER63732
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry

Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (ER63732) at 1/2,000 dilution. Lane 1: NCI-H226 cell lysate (20 µg/Lane) Lane 2: LoVo cell lysate (20 µg/Lane) Predicted band size: 54 kDa Observed band size: 70-100 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63732) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (ER63732) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IFNGR1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-100 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER63732) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of LoVo (positive) and MCF7 (low expression) labeling IFNGR1 with Rabbit anti-IFNGR1 antibody (ER63732) at 1/3,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFNGR1 antibody (ER63732) at 1/3,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 330 USD
to the supplier
IFNGR1
HUABIO
catalog: HA721952
domestic rabbit monoclonal (JE40-50)
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section

Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA721952) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate (20 µg/Lane) Lane 2: LoVo cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: THP-1 cell lysate (20 µg/Lane) Lane 5: K-562 cell lysate (20 µg/Lane) Lane 6: HEK-293 cell lysate (20 µg/Lane) Lane 7: MCF7 cell lysate (low expression) (20 µg/Lane) Lane 8: HL-60 cell lysate (20 µg/Lane) Lane 9: SW620 cell lysate (20 µg/Lane) Lane 10: Mouse liver tissue lysate (40 µg/Lane) Lane 11: Mouse thymus tissue lysate (40 µg/Lane) Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721952) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA721952) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IFNGR1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: 180 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721952) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IFNGR1 antibody (HA721952) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721952) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 385.00 USD
to the supplier
IFNGR1
HUABIO
catalog: HA722508
domestic rabbit monoclonal (PSH06-11)
reactivity: human
application: western blot

Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA722508) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate Lane 2: LoVo cell lysate Lane 3: HepG2 cell lysate Lane 4: THP-1 cell lysate Lane 5: MCF7 cell lysate (low expression) Lane 6: K-562 cell lysate Lane 7: SW620 cell lysate Lane 8: HL-60 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: Lane 1: 20 seconds; Lane 2-8: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722508) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of LoVo (positive) and MCF7 (low expression) labeling IFNGR1 with Rabbit anti-IFNGR1 antibody (HA722508) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFNGR1 antibody (HA722508) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA722508) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IFNGR1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 45/120 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722508) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
Human IFNGR1
HUABIO
catalog: HA722679
domestic rabbit monoclonal (PSH06-43)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human IFNGR1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722679) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human IFNGR1 protein (HA210514) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA722680, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IFNGR1 in MDA-MB-231 and MCF7 extract samples based on a 1,000 µg/ml extract load. The concentrations of IFNGR1 were measured in duplicates, interpolated from the IFNGR1 standard curve and corrected for sample dilution. Undiluted samples are MDA-MB-231 extract 50% and MCF7 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFNGR1 concentration was determined to be 2,358 pg/ml in MDA-MB-231 extract and 1,055 pg/ml in MCF7 extract.

Interpolated concentrations of spiked IFNGR1 in human cell culture media samples. The concentrations of IFNGR1 were measured in duplicates, interpolated from the IFNGR1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human IFNGR1
HUABIO
catalog: HA722680
domestic rabbit monoclonal (PSH06-44)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human IFNGR1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722679) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human IFNGR1 protein (HA210514) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA722680, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IFNGR1 in MDA-MB-231 and MCF7 extract samples based on a 1,000 µg/ml extract load. The concentrations of IFNGR1 were measured in duplicates, interpolated from the IFNGR1 standard curve and corrected for sample dilution. Undiluted samples are MDA-MB-231 extract 50% and MCF7 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFNGR1 concentration was determined to be 2,358 pg/ml in MDA-MB-231 extract and 1,055 pg/ml in MCF7 extract.

Interpolated concentrations of spiked IFNGR1 in human cell culture media samples. The concentrations of IFNGR1 were measured in duplicates, interpolated from the IFNGR1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
Human IFNGR1
HUABIO
catalog: HA722681B
domestic rabbit monoclonal (PSH06-44)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human IFNGR1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722679) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human IFNGR1 protein (HA210514) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA722681B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IFNGR1 in MDA-MB-231 and MCF7 extract samples based on a 1,000 µg/ml extract load. The concentrations of IFNGR1 were measured in duplicates, interpolated from the IFNGR1 standard curve and corrected for sample dilution. Undiluted samples are MDA-MB-231 extract 50% and MCF7 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFNGR1 concentration was determined to be 2,358 pg/ml in MDA-MB-231 extract and 1,055 pg/ml in MCF7 extract.

Interpolated concentrations of spiked IFNGR1 in human cell culture media samples. The concentrations of IFNGR1 were measured in duplicates, interpolated from the IFNGR1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 409.00 USD
to the supplier
IFNGR1
HUABIO
catalog: HA751049
domestic rabbit monoclonal (PSH06-11)
reactivity: human
application: western blot

Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA751049) at 1/1,000 dilution. Lane 1: NCI-H226 cell lysate Lane 2: LoVo cell lysate Lane 3: HepG2 cell lysate Lane 4: THP-1 cell lysate Lane 5: MCF7 cell lysate (low expression) Lane 6: K-562 cell lysate Lane 7: SW620 cell lysate Lane 8: HL-60 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 40-100 kDa Exposure time: Lane 1: 20 seconds; Lane 2-8: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751049) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry analysis of LoVo (positive) and MCF7 (low expression) labeling IFNGR1 with Rabbit anti-IFNGR1 antibody (HA751049) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IFNGR1 antibody (HA751049) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Western blot analysis of IFNGR1 on different lysates with Rabbit anti-IFNGR1 antibody (HA751049) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IFNGR1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 45/120 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751049) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier