Interferon gamma
HUABIO
catalog: ET1703-17

domestic rabbit monoclonal (JM10-10)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of Interferon gamma on different lysates with Rabbit anti-Interferon gamma antibody (ET1703-17) at 1/1,000 dilution. Lane 1: Human IFN-gamma recombinant protein, 50ng/Lane Lane 2: Mouse IFN-gamma recombinant protein, 50ng/Lane Lane 3: Rat IFN-gamma recombinant protein, 50ng/Lane Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-17) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of Interferon gamma was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1703-17, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier

Human Interferon gamma
HUABIO
catalog: HA721574

domestic rabbit monoclonal (PS01-75)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Interferon gamma matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721574) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Human Interferon gamma protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721575, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Interferon gamma
HUABIO
catalog: HA721575

domestic rabbit monoclonal (PS01-76)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human Interferon gamma matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721574) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Human Interferon gamma protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721575, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Interferon gamma
HUABIO
catalog: HA721576

domestic rabbit monoclonal (PS01-76)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of Human Interferon gamma matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721574) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Human Interferon gamma protein starting from 800 pg/ml to 0 pg/ml and detect antibody (HA721575, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 409.00 USD
to the supplier

Human Interferon gamma
HUABIO
catalog: HA721683

domestic rabbit monoclonal (PSH01-45)
reactivity: human
application: ELISA

Standard curve of human IFNG matched pair antibodies: Capture: HA721683, Interferon gamma Rabbit mAb [PSH01-45] Detector: HA721685, Interferon gamma Rabbit mAb [PSH01-47] Sandwich ELISA analysis of human IFNG matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH01-45] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IFNG protein starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH01-47]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IFN-gamma in human PBMC cell culture supernatant. Capture: HA721683, Interferon gamma Rabbit mAb [PSH01-45] Detector: HA721685, Interferon gamma Rabbit mAb [PSH01-47] PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 48 hours. The concentrations of IFN-gamma were interpolated from the IFN-gamma standard curves and corrected for sample dilution. Undiluted samples are PHA-M stimulated PBMC supernatant 10% and unstimulated PBMC supernatant 10%. The mean IFN-gamma concentration was determined to be 12,543 pg/ml in PHA-M stimulated PBMC supernatant and undetectable in the unstimulated PBMC supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Interferon gamma
HUABIO
catalog: HA721684

domestic rabbit monoclonal (PSH01-46)
reactivity: human
application: ELISA

Standard curve of human IFNG matched pair antibodies: Sandwich ELISA analysis of human IFNG matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH01-46] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IFNG protein starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH01-47]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Interferon gamma
HUABIO
catalog: HA721685

domestic rabbit monoclonal (PSH01-47)
reactivity: human
application: ELISA

Standard curve of human IFNG matched pair antibodies: Capture: HA721683, Interferon gamma Rabbit mAb [PSH01-45] Detector: HA721685, Interferon gamma Rabbit mAb [PSH01-47] Sandwich ELISA analysis of human IFNG matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721683) diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted IFNG protein starting from 2500 pg/ml to 0 pg/ml and detect antibody (HA721685, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Standard curve of human IFNG matched pair antibodies: Sandwich ELISA analysis of human IFNG matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH01-46] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IFNG protein starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH01-47]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IFN-gamma in human PBMC cell culture supernatant. Capture: HA721683, Interferon gamma Rabbit mAb [PSH01-45] Detector: HA721685, Interferon gamma Rabbit mAb [PSH01-47] PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 48 hours. The concentrations of IFN-gamma were interpolated from the IFN-gamma standard curves and corrected for sample dilution. Undiluted samples are PHA-M stimulated PBMC supernatant 10% and unstimulated PBMC supernatant 10%. The mean IFN-gamma concentration was determined to be 12,543 pg/ml in PHA-M stimulated PBMC supernatant and undetectable in the unstimulated PBMC supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Interferon gamma
HUABIO
catalog: HA722645B

domestic rabbit monoclonal (PSH01-47)
reactivity: human
conjugate: biotin
application: ELISA

Standard curve of human IFNG matched pair antibodies: Capture: HA721683, Interferon gamma Rabbit mAb [PSH01-45] Detector: HA721685, Interferon gamma Rabbit mAb [PSH01-47] Sandwich ELISA analysis of human IFNG matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH01-45] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IFNG protein starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH01-47]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native IFN-gamma in human PBMC cell culture supernatant. Capture: HA721683, Interferon gamma Rabbit mAb [PSH01-45] Detector: HA721685, Interferon gamma Rabbit mAb [PSH01-47] PBMC cells were stimulated with 10 µg/ml PHA-M or vehicle control and incubated for 48 hours. The concentrations of IFN-gamma were interpolated from the IFN-gamma standard curves and corrected for sample dilution. Undiluted samples are PHA-M stimulated PBMC supernatant 10% and unstimulated PBMC supernatant 10%. The mean IFN-gamma concentration was determined to be 12,543 pg/ml in PHA-M stimulated PBMC supernatant and undetectable in the unstimulated PBMC supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

Interferon gamma
HUABIO
catalog: HA750373

domestic rabbit monoclonal (JM10-10)
reactivity: human
application: western blot, flow cytometry

Western blot analysis of Interferon gamma on different lysates with Rabbit anti-Interferon gamma antibody (HA750373) at 1/1,000 dilution. Lane 1: Human IFN-gamma recombinant protein, 50ng/Lane Lane 2: Mouse IFN-gamma recombinant protein, 50ng/Lane Lane 3: Rat IFN-gamma recombinant protein, 50ng/Lane Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750373) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

Flow cytometric analysis of Interferon gamma was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (HA750373, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier