domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
All lanes: Western blot analysis of HMGB1 with anti-HMGB1 antibody (ER0913) at 1/500 dilution. Lane 1: Wild-type Raw264.7 whole cell lysate. Lane 2: HMGB1 knockdown Raw264.7 whole cell lysate. ER0913 was shown to specifically react with HMGB1 in Wild-type Raw264.7 cells. Weakened band was observed when HMGB1 knockdown sample was tested. Wild-type and HMGB1 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-HMGB1 antibody (ER0913, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Western blot analysis of HMGB1 on different cell lysates using anti- HMGB1 antibody at 1/500 dilution. Positive control: Lane 1: HepG2 cell lysate Lane 2: MCF-7 cell lysate Lane 3: F9 cell lysate Lane 4: A549 cell lysate
ICC staining HMGB1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (SA39-03)
reactivity: human, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of HMGB1 on different lysates with Rabbit anti-HMGB1 antibody (ET1601-2) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: HCT 116 cell lysate Lane 4: A549 cell lysate Lane 5: Jurkat cell lysate Lane 6: C2C12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-2) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of HMGB1 with anti-HMGB1 antibody [SA39-03] (ET1601-2) at 1/50,000 dilution. Lane 1: Wild-type Raw264.7 whole cell lysate. Lane 2: HMGB1 knockout Raw264.7 whole cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-HMGB1 antibody (ET1601-2, 1/50,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Western blot analysis of HMGB1 on different lysates with Rabbit anti-HMGB1 antibody (ET1601-2) at 1/20,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: Mouse thymus tissue lysate (20 µg/Lane) Lane 7: Rat spleen tissue lysate (30 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-2) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 50μl
price: 205.00 USD
to the supplier
domestic rabbit monoclonal (SA39-03)
reactivity: human, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of HMGB1 on different lysates with Rabbit anti-HMGB1 antibody (HA750011) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: HCT 116 cell lysate Lane 4: A549 cell lysate Lane 5: Jurkat cell lysate Lane 6: C2C12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750011) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of HMGB1 with anti-HMGB1 antibody [SA39-03] (HA750011) at 1/50,000 dilution. Lane 1: Wild-type Raw264.7 whole cell lysate. Lane 2: HMGB1 knockout Raw264.7 whole cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-HMGB1 antibody (HA750011, 1/50,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Western blot analysis of HMGB1 on different lysates with Rabbit anti-HMGB1 antibody (HA750011) at 1/20,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: Jurkat cell lysate (20 µg/Lane) Lane 6: Mouse thymus tissue lysate (20 µg/Lane) Lane 7: Rat spleen tissue lysate (30 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750011) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
All lanes: Western blot analysis of HMGB1 with anti-HMGB1 antibody (R1309-12) at 1/500 dilution. Lane 1: Wild-type Raw264.7 whole cell lysate. Lane 2: HMGB1 knockout Raw264.7 whole cell lysate. R1309-12 was shown to specifically react with HMGB1 in wild-type Raw264.7 cells. No band was observed when HMGB1 knockout sample was tested. Wild-type and HMGB1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-HMGB1 antibody (R1309-12, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Western blot analysis on MCF-7 (1), PC12 (2) and F9 (3) cell lysates using anti-HMGB1 rabbit polyclonal antibodies.
Immunofluorescent staining of Hela cells using anti- HMGB1 rabbit polyclonal antibody.
quantity: 100μl
price: 330 USD
to the supplier