mouse monoclonal (C2-E4)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1701-15) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Mouse placenta tissue lysate (40 µg/Lane) Lane 4: Rat placenta tissue lysate (40 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-15) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1701-15) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IMP3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-15) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
IMP3 was immunoprecipitated from 0.2 mg 293T cell lysate with EM1701-15 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using EM1701-15 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T cell lysate (input) Lane 2: EM1701-15 IP in 293T cell lysate Lane 3: Mouse IgG instead of EM1701-15 in 293T cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds
quantity: 50μl
price: 205.00 USD
to the supplier
mouse monoclonal (4-C2-5)
reactivity: human, mouse, rat
application: western blot, flow cytometry
reactivity: human, mouse, rat
application: western blot, flow cytometry
Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1801-01) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (40 µg/Lane) Lane 5: Mouse placenta tissue lysate (40 µg/Lane) Lane 6: Rat spleen tissue lysate (40 µg/Lane) Lane 7: Rat placenta tissue lysate (40 µg/Lane) Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: Lane 1-4: 20 seconds; Lane 5-6: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-01) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IMP3 on different lysates with Mouse anti-IMP3 antibody (EM1801-01) at 1/5,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IMP3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1801-01) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of PANC-1 cells with IMP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (R1512-10) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-10) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IMP3 on different lysates with Rabbit anti-IMP3 antibody (R1512-10) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IMP3 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 64 kDa Observed band size: 64 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1512-10) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IMP-3 antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 330 USD
to the supplier
mouse monoclonal (C2-E4)
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section