Human HGF
HUABIO
catalog: HA721778
domestic rabbit monoclonal (PSH02-06)
reactivity: human
application: ELISA
quantity: 100μl
price: 649.00 USD
to the supplier
Human HGF
HUABIO
catalog: HA721779
domestic rabbit monoclonal (PSH02-07)
reactivity: human
application: ELISA
quantity: 100μl
price: 649.00 USD
to the supplier
Human HGF
HUABIO
catalog: HA722042
domestic rabbit monoclonal (PSH03-78)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human HGF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA722042 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human HGF protein (HA210739) starting from 5000 pg/ml to 0 pg/ml and detect antibody HA722043-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

The concentrations of Human HGF were interpolated from the HGF standard curves and corrected for sample dilution.The mean HGF concentration was determined to be 1,141 pg/ml in THP-1 cell supernatant.

The concentrations of Human HGF were interpolated from the HGF standard curves and corrected for sample dilution.The mean HGF concentration was determined to be 1,657 pg/ml in mixed human serum from twenty volunteers.
quantity: 100μl
price: 649.00 USD
to the supplier
Human HGF
HUABIO
catalog: HA722043
domestic rabbit monoclonal (PSH03-79)
reactivity: human
application: ELISA

Sandwich ELISA analysis of Human HGF matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA722042 diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human HGF protein (HA210739) starting from 5000 pg/ml to 0 pg/ml and detect antibody HA722043-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

The concentrations of Human HGF were interpolated from the HGF standard curves and corrected for sample dilution.The mean HGF concentration was determined to be 1,141 pg/ml in THP-1 cell supernatant.

The concentrations of Human HGF were interpolated from the HGF standard curves and corrected for sample dilution.The mean HGF concentration was determined to be 1,657 pg/ml in mixed human serum from twenty volunteers.
quantity: 100μl
price: 649.00 USD
to the supplier
Human HGF
HUABIO
catalog: HA722619B
domestic rabbit monoclonal (PSH03-79)
reactivity: human
conjugate: biotin
application: ELISA
quantity: 100μl
price: 409.00 USD
to the supplier
Human HGF
HUABIO
catalog: HA722640B
domestic rabbit monoclonal (PSH02-07)
reactivity: human
conjugate: biotin
application: ELISA
quantity: 100μl
price: 409.00 USD
to the supplier
HGF
HUABIO
catalog: HA723896
domestic rabbit monoclonal (PSH03-79)
reactivity: human
application: western blot

Western blot analysis of HGF on different lysates with Rabbit anti-HGF antibody (HA723896) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: NCI-H1299 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723896) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
HGF
HUABIO
catalog: HA751614
domestic rabbit monoclonal (PSH03-79)
reactivity: human
application: western blot

Western blot analysis of HGF on different lysates with Rabbit anti-HGF antibody (HA751614) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: NCI-H1299 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751614) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier