domestic rabbit polyclonal
reactivity: human, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of IL-17 on IL-17 recombinant protein with Rabbit anti-IL-17 antibody (ER1706-91) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Predicted band size: 18 kDa Observed band size: 16 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-91) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IL-17 antibody (ER1706-91) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-91) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of Jurkat cells with IL-17 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of IL-17A on recombinant protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-37, 1/20,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IL-17A on Rat stomach tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IL-17A on Human skeletal muscle tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH05-35)
reactivity: mouse, rat
application: ELISA
reactivity: mouse, rat
application: ELISA
Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722264) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-17A protein (HA210791) starting from 800 pg/ml to 0 pg/ml and detect antibody (HA722265, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Mouse lung were stimulated with 5 µg/mL LPS and incubated for 6 days. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution.Undiluted samples are LPS stimulated mouse lung supernatants 50% and unstimulated mouse lung supernatants 50%. The mean IL17A concentration was determined to be 621 pg/mL in LPS stimulated mouse lung supernatant and undetectable in the unstimulated mouse lung supernatant.
Interpolated concentrations of native IL17A in unstimulated or stimulated rat spleen culture supernatant. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Rat spleen were stimulated with 5 µg/mL Concanavalin A and incubated for 2 days.The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution. Undiluted samples are Concanavalin A stimulated rat spleen supernatants 20% and unstimulated rat spleen supernatants 50%. The mean IL17A concentration was determined to be 3,703 pg/mL in Concanavalin A stimulated rat spleen supernatant and undetectable in the unstimulated rat spleen supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-36)
reactivity: mouse, rat
application: ELISA
reactivity: mouse, rat
application: ELISA
Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722264) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-17A protein (HA210791) starting from 800 pg/ml to 0 pg/ml and detect antibody (HA722265, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Mouse lung were stimulated with 5 µg/mL LPS and incubated for 6 days. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution.Undiluted samples are LPS stimulated mouse lung supernatants 50% and unstimulated mouse lung supernatants 50%. The mean IL17A concentration was determined to be 621 pg/mL in LPS stimulated mouse lung supernatant and undetectable in the unstimulated mouse lung supernatant.
Interpolated concentrations of native IL17A in unstimulated or stimulated rat spleen culture supernatant. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Rat spleen were stimulated with 5 µg/mL Concanavalin A and incubated for 2 days.The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution. Undiluted samples are Concanavalin A stimulated rat spleen supernatants 20% and unstimulated rat spleen supernatants 50%. The mean IL17A concentration was determined to be 3,703 pg/mL in Concanavalin A stimulated rat spleen supernatant and undetectable in the unstimulated rat spleen supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-36)
reactivity: mouse, rat
conjugate: biotin
application: ELISA
reactivity: mouse, rat
conjugate: biotin
application: ELISA
Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722264) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-17A protein (HA210791) starting from 800 pg/ml to 0 pg/ml and detect antibody (HA722265, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Mouse lung were stimulated with 5 µg/mL LPS and incubated for 6 days. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution.Undiluted samples are LPS stimulated mouse lung supernatants 50% and unstimulated mouse lung supernatants 50%. The mean IL17A concentration was determined to be 621 pg/mL in LPS stimulated mouse lung supernatant and undetectable in the unstimulated mouse lung supernatant.
Interpolated concentrations of native IL17A in unstimulated or stimulated rat spleen culture supernatant. Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35] Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36] Rat spleen were stimulated with 5 µg/mL Concanavalin A and incubated for 2 days.The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution. Undiluted samples are Concanavalin A stimulated rat spleen supernatants 20% and unstimulated rat spleen supernatants 50%. The mean IL17A concentration was determined to be 3,703 pg/mL in Concanavalin A stimulated rat spleen supernatant and undetectable in the unstimulated rat spleen supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier