Human Granzyme A
HUABIO
catalog: HA723092

domestic rabbit monoclonal (PSH09-34)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Granzyme A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723092) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Granzyme A protein (HA210916) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723093, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Granzyme A in NK-92 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are NK-92 cell culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 137.0 ng/ml in NK-92 cell culture supernatant.

Interpolated concentrations of native Granzyme A in Jurkat and HL-60 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are Jurkat cell culture supernatant 100% and HL-60 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 1,184.5 pg/ml in Jurkat cell culture supernatant and undetectable in HL-60 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Granzyme A
HUABIO
catalog: HA723093

domestic rabbit monoclonal (PSH09-35)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Granzyme A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723092) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Granzyme A protein (HA210916) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723093, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Granzyme A in NK-92 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are NK-92 cell culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 137.0 ng/ml in NK-92 cell culture supernatant.

Interpolated concentrations of native Granzyme A in Jurkat and HL-60 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are Jurkat cell culture supernatant 100% and HL-60 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 1,184.5 pg/ml in Jurkat cell culture supernatant and undetectable in HL-60 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Granzyme A
HUABIO
catalog: HA723094B

domestic rabbit monoclonal (PSH09-35)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human Granzyme A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723092) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Granzyme A protein (HA210916) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723094B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Granzyme A in NK-92 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are NK-92 cell culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 137.0 ng/ml in NK-92 cell culture supernatant.

Interpolated concentrations of native Granzyme A in Jurkat and HL-60 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are Jurkat cell culture supernatant 100% and HL-60 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 1,184.5 pg/ml in Jurkat cell culture supernatant and undetectable in HL-60 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

Human Granzyme A
HUABIO
catalog: HA723095

domestic rabbit monoclonal (PSH09-36)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Granzyme A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723095) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Granzyme A protein (HA210916) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723096, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Granzyme A in NK-92 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are NK-92 cell culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 158.2 ng/ml in NK-92 cell culture supernatant.

Interpolated concentrations of native Granzyme A in Jurkat and HL-60 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are Jurkat cell culture supernatant 100% and HL-60 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 1,633.2 pg/ml in Jurkat cell culture supernatant and undetectable in HL-60 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Granzyme A
HUABIO
catalog: HA723096

domestic rabbit monoclonal (PSH09-37)
reactivity: human
application: ELISA

Sandwich ELISA analysis of human Granzyme A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723095) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Granzyme A protein (HA210916) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723096, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Granzyme A in NK-92 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are NK-92 cell culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 158.2 ng/ml in NK-92 cell culture supernatant.

Interpolated concentrations of native Granzyme A in Jurkat and HL-60 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are Jurkat cell culture supernatant 100% and HL-60 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 1,633.2 pg/ml in Jurkat cell culture supernatant and undetectable in HL-60 cell culture supernatant.
quantity: 100μl
price: 649.00 USD
to the supplier

Human Granzyme A
HUABIO
catalog: HA723097B

domestic rabbit monoclonal (PSH09-37)
reactivity: human
conjugate: biotin
application: ELISA

Sandwich ELISA analysis of human Granzyme A matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA723095) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Granzyme A protein (HA210916) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA723097B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Granzyme A in NK-92 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are NK-92 cell culture supernatant 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 158.2 ng/ml in NK-92 cell culture supernatant.

Interpolated concentrations of native Granzyme A in Jurkat and HL-60 cell culture supernatant. The concentrations of Granzyme A were measured in duplicates, interpolated from the Granzyme A standard curve and corrected for sample dilution. Undiluted samples are Jurkat cell culture supernatant 100% and HL-60 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme A concentration was determined to be 1,633.2 pg/ml in Jurkat cell culture supernatant and undetectable in HL-60 cell culture supernatant.
quantity: 100μl
price: 409.00 USD
to the supplier

Granzyme A
HUABIO
catalog: HA723633

domestic rabbit monoclonal (PSH14-39)
reactivity: human
application: western blot

Western blot analysis of Granzyme A on different lysates with Rabbit anti-Granzyme A antibody (HA723633) at 1/25,000 dilution. Lane 1: NK-92 cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723633) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Granzyme A on different lysates with Rabbit anti-Granzyme A antibody (HA723633) at 1/10,000 dilution. Lane 1: Human Granzyme A recombinant protein Lane 2: Human Granzyme B recombinant protein Lane 3: Human Granzyme K recombinant protein Lysates/proteins at 20 ng/Lane. Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723633) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier

Granzyme A
HUABIO
catalog: HA751521

domestic rabbit monoclonal (PSH14-39)
reactivity: human
application: western blot

Western blot analysis of Granzyme A on different lysates with Rabbit anti-Granzyme A antibody (HA751521) at 1/25,000 dilution. Lane 1: NK-92 cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751521) at 1/25,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Granzyme A on different lysates with Rabbit anti-Granzyme A antibody (HA751521) at 1/10,000 dilution. Lane 1: Human Granzyme A recombinant protein Lane 2: Human Granzyme B recombinant protein Lane 3: Human Granzyme K recombinant protein Lysates/proteins at 20 ng/Lane. Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751521) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier