domestic rabbit monoclonal (PSH05-94)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human CXCL1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722493) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant standard Human CXCL1/GRO alpha protein (HA210838) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722494, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CXCL1 in human PBMC culture supernatant samples. Human PBMCs were cultured for 5 days at 37˚C in the presence or absence of 10ug/ml PHA-M. The concentrations of CXCL1 were interpolated from the standard curve and corrected for sample dilution. The mean CXCL1 concentration was determined to be 32pg/mL in unstimulated PBMC supernatants, and 1745 pg/mL in stimulated PBMC supernatants.
Interpolated concentrations of spiked CXCL1 in human cell culture media samples. The concentrations of CXCL1 were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-95)
reactivity: human
application: ELISA
reactivity: human
application: ELISA
Sandwich ELISA analysis of human CXCL1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722493) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant standard Human CXCL1/GRO alpha protein (HA210838) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722494, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CXCL1 in human PBMC culture supernatant samples. Human PBMCs were cultured for 5 days at 37˚C in the presence or absence of 10ug/ml PHA-M. The concentrations of CXCL1 were interpolated from the standard curve and corrected for sample dilution. The mean CXCL1 concentration was determined to be 32pg/mL in unstimulated PBMC supernatants, and 1745 pg/mL in stimulated PBMC supernatants.
Interpolated concentrations of spiked CXCL1 in human cell culture media samples. The concentrations of CXCL1 were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-95)
reactivity: human
conjugate: biotin
application: ELISA
reactivity: human
conjugate: biotin
application: ELISA
domestic rabbit monoclonal (PSH07-53)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of CXCL1 / GRO alpha on different lysates with Rabbit anti-CXCL1 / GRO alpha antibody (HA722841) at 1/2,000 dilution. Lane 1: HUVEC cell lysate Lane 2: HUVEC treated with 0.5μg/mL LPS for 24 hours cell lysate, then add 300ng/mL BFA for 20 hours cell lysate Lane 3: EA.hy926 cell lysate Lane 4: EA.hy926 treated with 0.5μg/mL LPS for 4 hours cell lysate, then add 300ng/mL BFA for 20 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722841) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH07-53)
reactivity: human
application: western blot
reactivity: human
application: western blot
Western blot analysis of CXCL1 / GRO alpha on different lysates with Rabbit anti-CXCL1 / GRO alpha antibody (HA751174) at 1/2,000 dilution. Lane 1: HUVEC cell lysate Lane 2: HUVEC treated with 0.5μg/mL LPS for 24 hours cell lysate, then add 300ng/mL BFA for 20 hours cell lysate Lane 3: EA.hy926 cell lysate Lane 4: EA.hy926 treated with 0.5μg/mL LPS for 4 hours cell lysate, then add 300ng/mL BFA for 20 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722841) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier