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Mannose Receptor(CD206)
HUABIO
catalog: HA722892
domestic rabbit monoclonal (PSH07-76)
reactivity:
mouse
,
rat
application:
western blot
,
immunoprecipitation
,
immunohistochemistry - paraffin section
,
immunohistochemistry - frozen section
Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate (Macrophage M2 polarization, positive) Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate (Macrophage M1 polarization, negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 165 kDa Observed band size: 200 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722892) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA722892) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 165 kDa Observed band size: 200 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722892) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier
Mannose Receptor(CD206)
HUABIO
catalog: HA751188
domestic rabbit monoclonal (PSH07-76)
reactivity:
mouse
,
rat
application:
western blot
,
immunoprecipitation
,
immunohistochemistry - paraffin section
,
immunohistochemistry - frozen section
Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA751188) at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate (Macrophage M2 polarization, positive) Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate (Macrophage M1 polarization, negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 165 kDa Observed band size: 200 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751188) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Mannose Receptor(CD206) on different lysates with Rabbit anti-Mannose Receptor(CD206) antibody (HA751188) at 1/1,000 dilution. Lane 1: Mouse lung tissue lysate (20 µg/Lane) Lane 2: Mouse spleen tissue lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 165 kDa Observed band size: 200 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751188) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier
Mannose Receptor(CD206)
HUABIO
catalog: HA601452
domestic rabbit polyclonal (PSH07-76)
reactivity:
mouse
,
rat
application:
western blot
,
immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Western blot analysis of Mannose Receptor(CD206) on different lysates with Guinea pig anti-Mannose Receptor(CD206) antibody (HA601452) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate (20 µg/Lane) Lane 2: Mouse liver tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 165 kDa Observed band size: 200 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601452) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 360.00 USD
to the supplier
Mannose Receptor(CD206)
HUABIO
catalog: HA601453
domestic rabbit polyclonal (PSH07-76)
reactivity:
mouse
,
rat
application:
western blot
,
immunohistochemistry - paraffin section
,
immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Application: IF-Tissue Species: Mouse Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/500
quantity: 100μl
price: 360.00 USD
to the supplier
Mannose Receptor(CD206)
HUABIO
catalog: HA610286
domestic rabbit polyclonal (PSH07-76)
reactivity:
mouse
,
rat
application:
western blot
,
immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Western blot analysis of Mannose Receptor(CD206) on different lysates with Guinea pig anti-Mannose Receptor(CD206) antibody (HA610286) at 1/2,000 dilution. Lane 1: Mouse lung tissue lysate (20 µg/Lane) Lane 2: Mouse liver tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 165 kDa Observed band size: 200 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610286) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.
quantity: 100μg
price: 649.00 USD
to the supplier
Mannose Receptor(CD206)
HUABIO
catalog: HA610287
domestic rabbit polyclonal (PSH07-76)
reactivity:
mouse
,
rat
application:
western blot
,
immunohistochemistry - paraffin section
,
immunohistochemistry - frozen section
Application: IHC-Fr Species: Mouse Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Application: IHC-Fr Species: Rat Site: liver Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
Application: IF-Tissue Species: Mouse Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/500
quantity: 100μg
price: 649.00 USD
to the supplier