mouse monoclonal (3F1)
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Glucocorticoid Receptor alpha on different lysates with Mouse anti-Glucocorticoid Receptor alpha antibody (EM1701-65) at 1/5,000 dilution. Lane 1: SiHa cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-65) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Glucocorticoid Receptor alpha with Mouse anti-Glucocorticoid Receptor alpha antibody (EM1701-65) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Glucocorticoid Receptor alpha antibody (EM1701-65) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 360.00 USD
to the supplier
mouse monoclonal (3F2)
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section
reactivity: human, mouse
application: western blot, flow cytometry, immunohistochemistry - paraffin section
Western blot analysis of Glucocorticoid Receptor alpha on different lysates with Mouse anti-Glucocorticoid Receptor alpha antibody (EM1701-66) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-66) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.
quantity: 100μl
price: 360.00 USD
to the supplier
domestic rabbit monoclonal (JF0952)
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry
reactivity: human, mouse, rat, zebrafish
application: western blot, flow cytometry
Western blot analysis of Glucocorticoid Receptor on different lysates with Rabbit anti-Glucocorticoid Receptor antibody (ET1702-11) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C6 cell lysate Lane 5: Zebrafish tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 86 kDa Observed band size: 86 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-11) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling Glucocorticoid Receptor with Rabbit anti-Glucocorticoid Receptor antibody (ET1702-11) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucocorticoid Receptor antibody (ET1702-11) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Immunocytochemistry analysis of NIH/3T3 cells labeling Glucocorticoid Receptor with Rabbit anti-Glucocorticoid Receptor antibody (ET1702-11) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucocorticoid Receptor antibody (ET1702-11) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot
reactivity: human, mouse, rat
application: western blot
Western blot analysis of Glucocorticoid Receptor alpha on Hela cell lysate using anti-Glucocorticoid Receptor alpha antibody at 1/1,000 dilution.
ICC staining Glucocorticoid Receptor alpha in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Glucocorticoid Receptor alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
quantity: 100μl
price: 330 USD
to the supplier