domestic rabbit monoclonal (PSH05-47)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-CD68 antibody (HA722285) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722285, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of frozen mouse liver tissue with Rabbit anti-CD68 antibody (HA722285) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722285, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of frozen rat liver tissue with Rabbit anti-CD68 antibody (HA722285) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722285, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH06-65)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: M‑NFS‑60 cell lysate (20 µg/Lane) Lane 3: J774A.1 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (negative) (20 µg/Lane) Lane 5: Neuro-2a cell lysate (negative) (20 µg/Lane) Lane 6: Rat spleen tissue lysate (no heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 35 kDa Observed band size: 90-200 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA722737) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 60-100 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722737) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH05-47)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Immunofluorescence analysis of frozen mouse spleen tissue with Rabbit anti-CD68 antibody (HA751006) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751006, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of frozen mouse liver tissue with Rabbit anti-CD68 antibody (HA751006) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751006, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of frozen rat liver tissue with Rabbit anti-CD68 antibody (HA751006) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA751006, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH06-65)
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
reactivity: mouse, rat
application: western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA751094) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: M‑NFS‑60 cell lysate (20 µg/Lane) Lane 3: J774A.1 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (negative) (20 µg/Lane) Lane 5: Neuro-2a cell lysate (negative) (20 µg/Lane) Lane 6: Rat spleen tissue lysate (no heat) (40 µg/Lane) Notice: no heat means the lysate is not boiled. Predicted band size: 35 kDa Observed band size: 90-200 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751094) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD68 on different lysates with Rabbit anti-CD68 antibody (HA751094) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 60-100 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751094) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required
quantity: 100μl
price: 649.00 USD
to the supplier