domestic rabbit polyclonal
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
reactivity: human, mouse, rat
application: western blot, immunohistochemistry - paraffin section
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (ER1909-31) at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Mouse liver tissue lysate (40 µg/Lane) Lane 3: Mouse colon tissue lysate (40 µg/Lane) Lane 4: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 34 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1909-31) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Galectin 9 antibody (ER1909-31) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1909-31) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
quantity: 100μl
price: 330 USD
to the supplier
domestic rabbit monoclonal (PSH02-44)
reactivity: human, mouse
application: western blot, flow cytometry
reactivity: human, mouse
application: western blot, flow cytometry
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA721821) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: PC-3 cell lysate (low expression) Lane 3: PANC-1 cell lysate (low expression) Lane 4: HL-60 cell lysate Lane 5: A549 cell lysate Lane 6: Jurkat cell lysate Lane 7: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 40 kDa Observed band size: 36 kDa Exposure time: 2 minutes 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721821) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of THP-1 cells labeling Galectin 9. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721821, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH02-45)
reactivity: human, rat
application: western blot
reactivity: human, rat
application: western blot
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA721822) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: A549 cell lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 35 kDa Exposure time: 2 minutes 18 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721822) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 385.00 USD
to the supplier
domestic rabbit monoclonal (PSH02-44)
reactivity: human, mouse
application: western blot, flow cytometry
reactivity: human, mouse
application: western blot, flow cytometry
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA750809) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: PC-3 cell lysate (low expression) Lane 3: PANC-1 cell lysate (low expression) Lane 4: HL-60 cell lysate Lane 5: A549 cell lysate Lane 6: Jurkat cell lysate Lane 7: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 40 kDa Observed band size: 36 kDa Exposure time: 2 minutes 7 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750809) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of THP-1 cells labeling Galectin 9. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750809, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
quantity: 100μl
price: 649.00 USD
to the supplier
domestic rabbit monoclonal (PSH02-45)
reactivity: human, rat
application: western blot
reactivity: human, rat
application: western blot
Western blot analysis of Galectin 9 on different lysates with Rabbit anti-Galectin 9 antibody (HA750810) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: A549 cell lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 35 kDa Exposure time: 2 minutes 18 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750810) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
quantity: 100μl
price: 649.00 USD
to the supplier